Chloroquine dihydrochloride

Manufactured by Merck Group

Sourced in Sao Tome and Principe

Chloroquine dihydrochloride is a synthetic chemical compound used as a laboratory reagent. It is a dichloride salt of the organic compound chloroquine, which is commonly used as an antimalarial drug. As a laboratory chemical, chloroquine dihydrochloride is primarily used for research and analysis purposes, but its specific applications may vary depending on the research context.

Automatically generated - may contain errors

Lab products found in correlation

Profection kit, by Promega (2 mentions)

Close spelling variants of this product

Chloroquine

2 protocols using chloroquine dihydrochloride

Packaging and Transducing Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phoenix A (amphotropic) cells were plated at a density of 4 × 10⁵ cells/well on a 6-well plate. On the following day, culture media was replaced with fresh media (Dulbecco’s modified Eagle medium, high glucose, 10% fetal bovine serum, pen-strep, L-glutamine). Chloroquine dihydrochloride (S764663; Sigma, St. Louis, MO) was added to Phoenix cells 5 minutes before transfection and 2 μg of plasmid DNA was transfected into cells using the Profection kit (E1200; Promega, Madison, WI). The media was changed 10 hours after transfection. Retroviral supernatant was collected the following day and 2 mL was added to GP+E86 cells, plated at 4 × 10⁴ cells/well on a 6-well dish the previous day, after filtration (0.45 μm) and supplemented with protamine sulfate (5 μg/mL). GP+E86 cells were transduced again on the following day. Viral supernatant from GP+E86 cells was collected on the following 2 days and used to transduce C3H10T1/2 cells.

Tsai E.A., Gilbert M.A., Grochowski C.M., Underkoffler L.A., Meng H., Zhang X., Wang M.M., Shitaye H., Hankenson K.D., Piccoli D., Lin H., Kamath B.M., Devoto M., Spinner N.B, & Loomes K.M. (2016). THBS2 Is a Candidate Modifier of Liver Disease Severity in Alagille Syndrome. Cellular and Molecular Gastroenterology and Hepatology, 2(5), 663-675.e2.

+ Open protocol

+ Expand

Retroviral Transduction of C3H10T1/2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phoenix A (amphotropic) cells were plated at a density of 4 × 10⁵ cells/well on a 6-well plate. On the following day, culture media was replaced with fresh media (Dulbecco's modified Eagle medium, high glucose, 10% fetal bovine serum, pen-strep, L-glutamine). Chloroquine dihydrochloride (S764663; Sigma, St. Louis, MO) was added to Phoenix cells 5 minutes before transfection and 2 μg of plasmid DNA was transfected into cells using the Profection kit (E1200; Promega, Madison, WI). The media was changed 10 hours after transfection. Retroviral supernatant was collected the following day and 2 mL was added to GP+E86 cells, plated at 4 × 10⁴ cells/well on a 6-well dish the previous day, after filtration (0.45 μm) and supplemented with protamine sulfate (5 μg/mL). GP+E86 cells were transduced again on the following day. Viral supernatant from GP+E86 cells was collected on the following 2 days and used to transduce C3H10T1/2 cells.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!