Samples from gluteus maximus tissue in MSTN KO and WT rabbits (euthanized at 2 months of age) of F1 generation were homogenized and lysed in RIPA buffer supplemented with 2.5 μL/mL protease inhibitor cocktail (Roche) on ice for 30 min. The protein concentrations were determined by Bradford method (Bio-Rad). 35 μg of protein sample was subjected to a 5% stacking/12% separating SDS-polyacrylamide gel. The antibodies used in this study include: anti-MSTN polyclonal antibody (Abcam) and goat anti-rabbit IgG conjugated with horseradish peroxidase (HRP) (Santa Cruz, USA). The β-actin antibody (Santa Cruz) was used as an internal control.
The tissues of tongue and gluteus maximus from MSTN KO and WT rabbits (euthanized at 2 months old) of F1 generation were fixed with 4% paraformaldehyde, then embedded in paraffin wax and slide sectioned. The sections were stained with hematoxylin and eosin (H&E) and analyzed by microscope (Nikon ts100).
The tissues of tongue and gluteus maximus from MSTN KO and WT rabbits (euthanized at 2 months old) of F1 generation were fixed with 4% paraformaldehyde, then embedded in paraffin wax and slide sectioned. The sections were stained with hematoxylin and eosin (H&E) and analyzed by microscope (Nikon ts100).