Acquity uplc 1 class ultra high performance liquid chromatography system

To identify the alteration in the bile acid profiles induced by biliary diversion, we measured and identified 18 bile acids in plasma samples collected from GK rats before surgery and 8 weeks postoperatively through targeted metabolomics analysis. At postoperative week 8, all rats were gavaged with 10% ENSURE® (1 ml/100 g body weight (31 (link))) after fasting for 12 h. Blood was then collected from the angular vein at 2 hours after gavage. Targeted quantitative detection of serum bile acid was performed on an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC−MS) platform (Waters Corporation, Milford, MA, USA) using the Waters ACQUITY UPLC I-class ULTRA high-performance liquid chromatography system combined with the Waters XEVO TQ-S tandem quadrupole mass spectrometry system. Data acquisition was carried out with MassLynx v 4.1 software (Waters Corporation, Milford, MA, USA).

Chemical reference standards of cholic acid (DSTDD004201), chebulagic acid (DST200927-064), chebulic acid (DST200610-244), corilagin (DST210311-012), ethyl gallate (DSTDM006301), benzoic acid (DSTDB012001), linoleic acid (DST200611-211), betulin (DSTDB002701), aconitine (HR1001S4), benzoylmesaconine (DSTDB005601), benzoylaconitine (DSTDB005501), benzoylhypaconine (DSTDB005701), hypaconitine (DSTDC005801), mesaconitine (DSTDX002502), methyl caffeate (DST210427-054) and costunolide (DSTDM003001) were acquired from Chengdu Desite Biotechnology Co.,. (Chengdu, China), and the purity of all standards were above 98%. Ellagic acid (111959-201903, purity: HPLC ≥98%) was purchased from China National Institute for Standard Products and Drug Control (Beijing, China). Deoxyaconitine was bought from Chenguang Biotechnology Company (Chengdu, China). Agilent 7890A-5975C Gas chromatography-mass spectrometry and Agilent GC-MSD workstation were purchased from Agilent Technologies (Santa Clara, USA). ACQUITY UPLC I-Class Ultra High-Performance Liquid Chromatography system, Xevo G2-XS QTOF High-resolution time of flight mass spectrometer, MassLynxV 4.2 Data acquisition and analysis workstation were obtained from Waters Corporation (Milford, MA, United States).