Libraries were prepared using NEBNext Ultra II kits (NEB) following the manufacturer’s protocol. Following library preparation, ChIP samples were purified as described [30 (link)], while input samples were purified using a 2:1 ratio of AmpureXP (GE) beads to library. Following PCR amplification, all samples were purified using AMpure XP beads with 0.9:1 beads to PCR ratio. Samples were sequenced using the Illumina NextSeq 500 platform with paired ends. See S3 and S4 Tables for library statistics.
Ampurexp
Manufactured by GE Healthcare
The AmpureXP is a magnetic bead-based nucleic acid purification system designed for high-throughput purification of DNA, RNA, and proteins. It utilizes paramagnetic beads to capture, wash, and elute target biomolecules, providing a streamlined and efficient purification process.
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Lab products found in correlation
2 protocols using ampurexp
1
Illumina ChIP-seq Library Preparation
2
ChIP-seq Library Preparation and Sequencing
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Libraries were prepared using Next Ultra II kits (NEB) following the manufacturer's protocol. Following library preparation, ChIP samples were purified as described (Wal and Pugh, 2012) , while input samples were purified using a 2:1 ratio of AmpureXP (GE) beads to library. Following PCR amplification, all samples were purified using AMpure XP beads with 0.9:1 beads to PCR ratio. Samples were sequenced using the Illumina NextSeq 500 platform with paired ends.
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