Young adult animals cultivated overnight at 20°C were well-fed throughout, or starved by placing them on unseeded NGM agar plates and re-cultivating at 20°C for 3 hr prior to imaging. Animals were moved onto 2% agarose pads on a glass slide, anesthetized with 1.5 µl of 25 mM azide, and were covered with a cover glass prior to imaging. Images were taken using an AX70 fluorescence microscope (Olympus) with an Olympus 20X UPlanSApo lens (NA 0.75). The ROI in the anterior intestine was outlined within 48 µm (75 pixels) from the posterior end of the pharynx and mean pixel intensity was calculated following background subtraction using ImageJ (NIH). Confocal microscope images were obtained using a FV3000 microscope (Olympus) with an Olympus 40X UPFLN lens (NA 0.75), and were exported as hyperstack. tif files.
Upfln lens
Manufactured by Olympus
The 40X UPFLN lens is a high-quality optical component designed for use in various laboratory and scientific applications. It provides a magnification of 40X, allowing for detailed observation and analysis of samples. The lens is built with Olympus' renowned optical technology, ensuring accurate and clear images. However, a more detailed and unbiased description of its core function cannot be provided without the risk of extrapolation or interpretation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using upfln lens
1
Imaging Intestinal Fluorescence in C. elegans
2
Intestinal Lipid Imaging in C. elegans
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Young adult animals cultivated overnight at 20ºC were well-fed throughout, or starved by placing them on unseeded NGM agar plates and re-cultivating at 20°C for 3h prior to imaging. Animals were moved onto 2% agarose pads on a glass slide, anesthetized with 1.5 µl of 25 mM azide, and were covered with a cover glass prior to imaging. Images were taken using an AX70 fluorescence microscope (Olympus) with an Olympus 20X UPlanSApo lens (NA 0.75). The ROI in the anterior intestine was outlined within 48 µm (75 pixels) from the posterior end of the pharynx and mean pixel intensity was calculated following background subtraction using ImageJ (NIH). Confocal microscope images were obtained using a FV3000 microscope (Olympus) with an Olympus 40X UPFLN lens (NA 0.75), and were exported as hyperstack .tif files.
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