The apoptotic cells were evaluated on isolated Sertoli cells by the TUNEL assay using a commercial apoptosis detection kit (Terminal Deoxynucleotidyl Transferase Detection Kit; Promega, UK, Ltd). TUNEL analysis was performed to quantify cell apoptosis on isolated Sertoli cells a commercial apoptosis detection kit (Terminal Deoxynucleotidyl Transferase Detection Kit; Promega, UK, Ltd). Cells were cultured with 0, 5, 10 and 15 µg/mL AgNPs for 24 h, and then washed in PBS, fixed in 4% paraformaldehyde for 15 minutes, and incubated in a TUNEL reaction mixture for 1 hours at 37°C, according to the procedure as described previously (Habas et al., 2014) .
Habas K., Brinkworth M.H, & Anderson D. (2018). Silver nanoparticle-mediated cellular responses in isolated primary Sertoli cells in vitro. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 116(Pt B).
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