Mix and go competent cells

Manufactured by Zymo Research

Mix and Go competent cells are a type of laboratory equipment used for bacterial transformation. They are pre-made, chemically competent cells that are ready to be mixed with DNA samples and transformed, allowing for efficient uptake of genetic material into the bacterial cells.

Automatically generated - may contain errors

Lab products found in correlation

Kanamycin sulfate, by Merck Group (2 mentions) Trisodium citrate dehydrate, by Merck Group (2 mentions) Yeast extract, by Thermo Fisher Scientific (2 mentions) Tryptone, by Thermo Fisher Scientific (2 mentions) Sodium chloride (nacl), by Thermo Fisher Scientific (2 mentions) Tetracycline, by Merck Group (2 mentions) E coli er2738, by New England Biolabs (2 mentions) M13ke phage, by New England Biolabs (2 mentions) Gold 3 chloride trihydrate, by Merck Group (2 mentions) Cysteamine, by Merck Group (2 mentions) Qiaquick gel extraction kit, by Qiagen (2 mentions) Qiaprep spin miniprep kit, by Qiagen (2 mentions) N hydroxysuccinimide, by Merck Group (1 mentions) T4 dna ligase, by New England Biolabs (1 mentions) Ampicillin sodium salt, by Thermo Fisher Scientific (1 mentions) Sodium borohydride, by Thermo Fisher Scientific (1 mentions) Polyethylene glycol peg 8000, by Merck Group (1 mentions) Quickdetect e coliprotein ecp elisa kit, by Abcam (1 mentions) P aeruginosa, by Merck Group (1 mentions) N hydroxysuccinimide nhs, by Merck Group (1 mentions)

2 protocols using mix and go competent cells

Phage Display Bacterial Pathogen Detection

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagents were obtained
from the following
sources: gold(III) chloride trihydrate (HAuCl₃, 99.9%,
Sigma), sodium borohydride (NaBH₄, 98%, Fisher Scientific),
trisodium citrate dehydrate (99.9%, Sigma), E. coli (Migula) Castellani and Chalmers (ATCC27065, ATCC), Xanthomonas
campestris pv campestris (ATCC33913), X. campestris pv vesicatoria (ATCC35937), P. aeruginosa (Schroeter)
Migula (ATCC 25102), V. cholerae 0395 (donation from
Prof. Michael J. Mahan, UCSB), M13KE phage (NEB), M13-NotI-Kan construct,^{12 (link)} sodium chloride (NaCl, 99%, Fisher BioReagents),
tryptone (99%, Fisher BioReagents), yeast extract (99%, Fisher BioReagents),
human serum (from male AB clotted whole blood, Sigma), E.
coli ER2738 (NEB), fluorescein-5-maleimide (97%, TCI), N-(3-(dimethylamino)propyl)-N′-ethylcarbodiimide
hydrochloride (EDC, 99%, Sigma), N-hydroxysuccinimide
(NHS, 98%, Sigma), cysteamine (98%, Sigma), poly(ethylene glycol)
(PEG-8000, Sigma), dialysis kit (MWCO 3500 Da, Spectrum Laboratories),
tetracycline (Sigma), kanamycin sulfate (Sigma), Top 10F′ cyan
cells (Thermo Fisher), Mix and Go competent cells (Zymo Research),
QIAprep Spin Miniprep Kit (Qiagen), QIAquick Gel Extraction Kit (Qiagen),
and KpnI-HF/NotI-HF restriction enzyme and T4 DNA
ligase (NEB).

Peng H, & Chen I.A. (2018). Rapid Colorimetric Detection of Bacterial Species through the Capture of Gold Nanoparticles by Chimeric Phages. ACS Nano, 13(2), 1244-1252.

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Phage-based Biosensing Platform Development

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reagents were obtained from the following
sources: gold(III) chloride trihydrate (HAuCl₃, 99.9%,
Sigma), sodium borohydride (NaBH₄, 98%, Fisher Scientific),
trisodium citrate dehydrate (99.9%, Sigma), P. aeruginosa (Schroeter) Migula (ATCC25102), Vibrio cholerae 0395 (donation from Prof. Michael J. Mahan, UCSB), M13KE phage (NEB),
M13-NotI-Kan construct,^{39 (link)} sodium chloride (NaCl, 99%, Fisher BioReagents), tryptone (99%,
Fisher BioReagents), yeast extract (99%, Fisher BioReagents), E. coli ER2738 (NEB), N-(3-(dimethylamino)propyl)-N′-ethylcarbodiimide hydrochloride (EDC, 99%, Sigma), N-hydroxysuccinimide (NHS, 98%, Sigma), cysteamine (98%,
Sigma), poly(ethylene glycol) (PEG-8000, Sigma), dialysis kit (MWCO
3500 Da, Spectrum Laboratories), tetracycline (Sigma), thiol-PEG-acid
(HOOC-PEG-SH, PEG average M_n 5000 Da,
Sigma), kanamycin sulfate (Sigma), ampicillin sodium salt (Fisher
BioReagents), Mix and Go competent cells (Zymo Research), QIAprep
Spin Miniprep kit (Qiagen), QIAquick Gel Extraction kit (Qiagen), KpnI-HF/NotI-HF restriction enzyme and
T4 DNA ligase (NEB), and QuickDetect E. coli Protein (ECP) ELISA kit
(BioVision).

Peng H., Borg R.E., Nguyen A.B, & Chen I.A. (2020). Chimeric Phage Nanoparticles for Rapid Characterization of Bacterial Pathogens: Detection in Complex Biological Samples and Determination of Antibiotic Sensitivity. ACS Sensors, 5(5), 1491-1499.

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