Biorevo software

Manufactured by Keyence

BIOREVO software is a bioinformatics analysis tool developed by Keyence. It provides a platform for processing and analyzing biological data, such as DNA sequences, gene expression profiles, and protein structures. The software offers a range of functionalities to support researchers and scientists in their work, but a detailed description without interpretation or extrapolation cannot be provided.

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Lab products found in correlation

Bz 9000 microscope, by Keyence (3 mentions) Alexa fluor 488 and 555 conjugated secondary antibodies, by Thermo Fisher Scientific (2 mentions) Bromodeoxyuridine (brdu), by Agilent Technologies (2 mentions) Bromodeoxyuridine (brdu), by Nacalai Tesque (2 mentions) Vectastain elite abc kit, by Vector Laboratories (2 mentions) Lsm 510, by Zeiss (2 mentions) Diaminobenzidine substrate kit, by Vector Laboratories (1 mentions) Mca497, by Bio-Rad (1 mentions) Dab substrate kit, by Vector Laboratories (1 mentions) Bz 9000, by Keyence (1 mentions) Fluoview fv1000 d confocal laser scanning microscope, by Olympus (1 mentions)

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BIOREVO (32 citations)

4 protocols using biorevo software

Quantifying Pancreatic Beta-cell Proliferation

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Mice were intraperitoneally injected with BrdU (100 µg/g; Nacalai Tesque, Inc) for 3 days and sacrificed. Three pancreatic tissue sections (100 µm apart) from each animal were prepared by fixation and paraffin-embedding. The sections were immunostained with antibodies to insulin, muscarinic acetylcholine receptor M1 (Santa Cruz), BrdU (Dako), and muscarinic acetylcholine receptor M3 (Bioss). Biotinylated secondary antibodies, a Vectastain elite ABC kit and a diaminobenzidine substrate kit (Vector) were used to examine the sections under bright-field microscopy to determine the β-cell mass. Alexa Fluor 488- and 555-conjugated secondary antibodies (Invitrogen) were used for fluorescence microscopy analysis. All images were captured using a BZ-9000 microscope (Keyence). The percentage area of the pancreatic tissues occupied by β cells was calculated using BIOREVO software (Keyence)^{31 (link)}. In the BrdU staining experiment, 22–78 islets in each mouse were analysed to assess the frequency of BrdU-positive cells among the insulin-positive cells.

Ito Y., Kaji M., Sakamoto E, & Terauchi Y. (2019). The beneficial effects of a muscarinic agonist on pancreatic β-cells. Scientific Reports, 9, 16180.

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Quantifying Pancreatic β-cells and Macrophages

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Formalin-fixed, paraffin-embedded pancreas or adipose tissue sections were immunostained with antibodies to insulin (Santa Cruz, sc-9168), glucagon (Abcam, ab10988), or F4/80 (Serotec, MCA497). Biotinylated secondary antibodies, a VECTASTAIN elite ABC kit, and a DAB substrate kit (VECTOR) were used to examine the sections using bright-field microscopy, and Alexa Fluor 488- and 555-conjugated secondary antibodies (Invitrogen) were used for fluorescence microscopy. All the images were acquired using a BZ-9000 microscope (Keyence) or a Carl Zeiss LSM 510 confocal laser-scanning microscope. The percent area of the pancreatic tissue occupied by the β cells was calculated using BIOREVO software (Keyence), as described previously [22 (link)]. More than five tissue sections from each animal, including representative sections of each tissue region, were analyzed.

Shirakawa J., Okuyama T., Kyohara M., Yoshida E., Togashi Y., Tajima K., Yamazaki S., Kaji M., Koganei M., Sasaki H, & Terauchi Y. (2016). DPP-4 inhibition improves early mortality, β cell function, and adipose tissue inflammation in db/db mice fed a diet containing sucrose and linoleic acid. Diabetology & Metabolic Syndrome, 8, 16.

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LNA ISH with Tyramide Amplification

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RNA detection was performed according to de Planell-Saguer [37 (link)]. Specifically, the LNA ISH with following tyramide signal amplification protocol was used. CMK cells were prepared as cytospins from fresh mock cultures. All TAMRA-conjugated LNA probes were designed and synthesized by Exiqon. Fluorescence microscopy was carried out on a BZ9000 (Keyence), data analysis was performed with Biorevo Software (Keyence).

Emmrich S., Streltsov A., Schmidt F., Thangapandi V.R., Reinhardt D, & Klusmann J.H. (2014). LincRNAs MONC and MIR100HG act as oncogenes in acute megakaryoblastic leukemia. Molecular Cancer, 13, 171.

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Quantifying Pancreatic Beta Cell Proliferation

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Mice were injected intraperitoneally with BrdU (100 mg/kg; Nacalai Tesque, Kyoto, Japan); 5 h later, the pancreases were harvested for histological analyses. The dissected pancreases were processed and immunostained with antibodies to insulin (Santa Cruz, TX, USA) and BrdU (Dako, Tokyo, Japan). The beta cell mass and number of BrdU-positive cells were analysed as described previously [20 (link)]. All the images were acquired using a BZ-9000 microscope (Keyence, Osaka, Japan) or a FluoView FV1000-D confocal laser scanning microscope (Olympus, Tokyo, Japan). The per cent area of the pancreatic tissue occupied by beta cells was calculated using BIOREVO software (Keyence). In the BrdU immunostaining experiment, approximately 50 islets were analysed using WinROOF software (Mitani, Tokyo, Japan) to assess the proportion of immunostained nuclei among the insulin-positive cells in each mouse. Liver and adipose tissue samples were formalin-fixed, embedded in paraffin, sectioned and stained with haematoxylin and eosin. The white adipocyte areas were measured for 1000 or more cells per mouse in each of the groups using BIOREVO software.

Shirakawa J., Tajima K., Okuyama T., Kyohara M., Togashi Y., De Jesus D.F., Basile G., Kin T., Shapiro A.M., Kulkarni R.N, & Terauchi Y. (2020). Luseogliflozin increases beta cell proliferation through humoral factors that activate an insulin receptor- and IGF-1 receptor-independent pathway. Diabetologia, 63(3), 577-587.

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