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1 2 distearoyl sn glycero 3 phosphoethanolamine n succinyl polyethylene glycol 2000 dspe peg cooh

Manufactured by Avanti Polar Lipids
Sourced in United States

1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinyl(polyethylene glycol)-2000] (DSPE-PEG-COOH) is a phospholipid conjugate with a polyethylene glycol (PEG) and a carboxyl (COOH) group. It is a synthetic lipid molecule designed for use in various biotechnology and pharmaceutical applications.

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3 protocols using 1 2 distearoyl sn glycero 3 phosphoethanolamine n succinyl polyethylene glycol 2000 dspe peg cooh

1

Synthesis of Methotrexate-Loaded Liposomes

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1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-Hydroxysuccinimide (NHS) and Triethylamine (TEA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methotrexate (MTX) and NH2-PEG (1K) were bought by AlfaAesar (Haverhill, MA, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinyl(polyethylene glycol)-2000] (DSPE-PEG-COOH), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE-NH2) and 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Avanti Polar Lipid (Alabaster, AL, USA). All reagents and solvents were used without further purification. Cy5 was purchased from Luminoprobe (Hunt Valley, MD, USA).
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2

Curcumin-loaded PLGA-PEG Nanoparticles

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Poly(D, L-lactide-co-glycolide) acid-terminated (PLGA, lactide:glycolide 50:50, Mw 38,000–54,000), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Curcumin (Cur) was bought from AlfaAesar (Haverhill, MA, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinyl(polyethylene glycol)-2000] (DSPE-PEG-COOH) and 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) were purchased from Avanti Polar Lipid (Alabaster, AL, USA). All reagents and solvents were used without further purification.
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3

Methotrexate-Loaded PLGA Nanoparticles

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Poly(d,l-lactide-co-glycolide) acid terminated (PLGA, lactide:glycolide 50:50,
Mw 38 000–54 000), 1-ethyl-3-(3-(dimethylamino)propyl)
carbodiimide (EDC), N-hydroxysuccinimide (NHS), triethylamine
(TEA), low-density lipoproteins (LDL), and a cholesterol quantitation
kit were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methotrexate
(MTX) was bought by AlfaAesar (Haverhill, MA, USA). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[succinyl(polyethylene
glycol)-2000] (DSPE-PEG-COOH), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine
(DSPE_NH2), and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine
(DPPC) were purchased from Avanti Polar Lipid (Alabaster, AL, USA).
All reagents and solvents were used without further purification.
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