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Hcc1954 cell line

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The HCC1954 cell line is a human breast cancer cell line derived from a primary ductal carcinoma. It is a widely used model for breast cancer research. The cell line exhibits characteristics of human mammary epithelial cells and has been extensively studied for its molecular and cellular properties.

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Pgl4.51 luc2 cmv neo vector, by Promega (1 mentions)

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HCC1954 (183 citations) HCC1954 cells (8 citations)

3 protocols using hcc1954 cell line

1

Breast Cancer Cell Line Maintenance

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MCF10F, trMCF, and bsMCF were maintained in DMEM:F12 supplemented media (Appendix S1). bsMCF cells were transfected with pGL4.51(luc2/CMV/Neo) vector (Promega, San Luis Obispo, CA) and maintained in media with 800 μg/mL G418 (so‐called bsMCF‐luc cells). MCF10F, T47D, MCF7, SK‐BR‐3, MDA‐MB‐231, MDA‐MB‐468, and Hs578t were from cell culture facility of FCCC. HCC1954 cell line was from American Type Culture Collection (ATCC). Sum149pt and Sum159pt were obtained from Asterand (Detroit, MI), and the media used for these cells are described in Appendix S1. All cell lines used for this study were used in less than ten passages after recovery.
Su Y., Pogash T.J., Nguyen T.D, & Russo J. (2016). Development and characterization of two human triple‐negative breast cancer cell lines with highly tumorigenic and metastatic capabilities. Cancer Medicine, 5(3), 558-573.
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2

HCC1954 Cell Line Characterization

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HCC1954 cell line was obtained from American Type Culture Collection (ATCC) and maintained as recommended in RPMI media + 10% FBS at 37°C in 5% CO2. Cells were limited to under 20 passages for all experiments. HCC1954 is ER-/HER2+ but not TOP2A amplified, has a doubling time of ~40 hours and is readily transduced with lentiviral particles. Additionally, it is doxorubicin-sensitive and paclitaxel-sensitive, which is important for showing that KDM4B modulation specifically leads to changes in anthracycline response and not generally to small molecule inhibitors. These observations indicated that it might be a useful model for studying the relationship between response to therapeutics and CRGs.
Seoane J.A., Kirkland J.G., Caswell-Jin J.L., Crabtree G.R, & Curtis C. (2019). Chromatin Regulators Mediate Anthracycline Sensitivity in Breast Cancer. Nature medicine, 25(11), 1721-1727.
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3

HCC1954 Cell Line Characterization

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HCC1954 cell line was obtained from American Type Culture Collection (ATCC) and maintained as recommended in RPMI media + 10% FBS at 37°C in 5% CO2. Cells were limited to under 20 passages for all experiments. HCC1954 is ER-/HER2+ but not TOP2A amplified, has a doubling time of ~40 hours and is readily transduced with lentiviral particles. Additionally, it is doxorubicin-sensitive and paclitaxel-sensitive, which is important for showing that KDM4B modulation specifically leads to changes in anthracycline response and not generally to small molecule inhibitors. These observations indicated that it might be a useful model for studying the relationship between response to therapeutics and CRGs.
Seoane J.A., Kirkland J.G., Caswell-Jin J.L., Crabtree G.R, & Curtis C. (2019). Chromatin Regulators Mediate Anthracycline Sensitivity in Breast Cancer. Nature medicine, 25(11), 1721-1727.
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