Twenty-five micrograms of protein from ScAT were suspended in 6.3 μL of sample buffer and 2.5 μL 1 M DTT to run electrophoresis using 10% bis-tris gel (Life Technologies, Rockville, MD, USA). Proteins were transferred to a polyvinylidene difluoride membrane, washed with tris-buffered saline and hybridized with primary anti-EHMT2/G9A antibody ab185050 (Abcam, Cambridge, MA, USA), anti-E4BP4 antibody sc-74415 (Santa Cruz, Dallas, TX, USA), anti-KDM4C NBP1–49600 (Novus Biologicals LLC, Centennial, CO, USA) and normalized to antibeta-actin ab8226 (Abcam, Cambridge, MA, USA) or anti-GAPDH antibody ab181602 (Abcam, Cambridge, MA, USA) 4 °C overnight. The membrane was washed with 1 × PBS containing 0.1% Tween and hybridized with a secondary antibody (goat antimouse 1:10,000 and goat antirabbit 1:10,000) for 1 h in buffer containing 0.1% Tween and 0.01% SDS. Images were developed using an Odyssey Infrared Imager (Li-Cor Biosciences Inc., Lincoln, NE, USA).
Anti kdm4c nbp1 49600
Manufactured by Novus Biologicals
Sourced in United States
Anti-KDM4C NBP1–49600 is a lab equipment product. It is an antibody that targets the KDM4C protein, which is involved in histone demethylation.
Automatically generated - may contain errors
2 protocols using anti kdm4c nbp1 49600
1
Western Blot Analysis of Epigenetic Regulators
2
Western Blot Analysis of Epigenetic Regulators
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Twenty-five micrograms of protein from ScAT were suspended in 6.3 μL of sample buffer and 2.5 μL 1 M DTT to run electrophoresis using 10% bis-tris gel (Life Technologies, Rockville, MD, USA). Proteins were transferred to a polyvinylidene difluoride membrane, washed with tris-buffered saline and hybridized with primary anti-EHMT2/G9A antibody ab185050 (Abcam, Cambridge, MA, USA), anti-E4BP4 antibody sc-74415 (Santa Cruz, Dallas, TX, USA), anti-KDM4C NBP1–49600 (Novus Biologicals LLC, Centennial, CO, USA) and normalized to antibeta-actin ab8226 (Abcam, Cambridge, MA, USA) or anti-GAPDH antibody ab181602 (Abcam, Cambridge, MA, USA) 4 °C overnight. The membrane was washed with 1 × PBS containing 0.1% Tween and hybridized with a secondary antibody (goat antimouse 1:10,000 and goat antirabbit 1:10,000) for 1 h in buffer containing 0.1% Tween and 0.01% SDS. Images were developed using an Odyssey Infrared Imager (Li-Cor Biosciences Inc., Lincoln, NE, USA).
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