Immunolabeled overview preparations as well as histological preparations were viewed, analyzed and documented using either an Axioskop 2 compound light microscope (Zeiss) with a stem-mounted SpotFlex digital camera (Diagnostic Instruments) or an Axio Imager.A1 compound light microscope (Zeiss) with a ProgRes C14 plus digital camera (Jenoptik, Germany). In order to improve the depth of field, selectively focused images were stacked and combined using Helicon focus 4.2.7 software (Helicon Soft Ltd.) in some instances. Confocal imaging was performed using either a LSM 710 (Zeiss) or a CLSM 2 (Leica) confocal microscope, and 3D images were created using ImageJ (NIH).
Axioskop 2 compound light microscope
Manufactured by Zeiss
The Axioskop 2 is a compound light microscope manufactured by Zeiss. It is designed for high-resolution imaging and observation of samples. The Axioskop 2 features a stable optical system and a range of illumination options to support various research and analysis applications.
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Lab products found in correlation
2 protocols using axioskop 2 compound light microscope
1
Immunolabeled Protocol Imaging Workflow
2
Micromorphological Identification of Fungal Species
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In order to check if the molecular identification was compatible with phenotypic characteristics of each species, micromorphological characters were observed after 7-10 days of incubation on carnation leaf agar (CLA; sterile leaf pieces, 20 g Merck agar, 1000 mL H 2 O) [42] . All microscopic characters were examined using sterile water as a mounting medium with a Zeiss Axioskop 2 compound light microscope, including the shape of macroconidia produced in sporodochia, the shape and mode of formation of microconidia and chlamydospores on CLA. To facilitate the comparison of relevant micro-and macroconidial features, composite photo plates were assembled from separate photo micrographs using Adobe Photoshop CC [2] .
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