Veleta 4k ccd camera

The virion morphology of Paride was analyzed by transmission electron microscopy following common procedures in the field^{79 (link)}. Briefly, 5 μl drops of high-titer lysate were adsorbed to 400 mesh carbon-coated grids, which were rendered hydrophilic using a glow-discharger at low vacuum conditions. They were subsequently stained on 5 μl drops of 2% (w/v) uranyl acetate. Samples were examined using an FEI Tecnai G2 Spirit transmission electron microscope (FEI Company, Hillsboro, Oregon, USA) operating at 80-kV accelerating voltage. Images were recorded with a side-mounted Olympus Veleta CCD camera 4k using EMSIS RADIUS software at a nominal magnification of typically ×150,000.

The virion morphology of representative bacteriophages of the different taxonomic groups included in the BASEL collection was analyzed by transmission electron microscopy following common procedures in the field [149 (link)]. Briefly, 5 μl drops of high-titer lysates were adsorbed to 400 mesh carbon-coated grids, which were rendered hydrophilic using a glow-discharger at low vacuum conditions. They were subsequently stained on 5 μl drops of 2% (w/v) uranyl acetate. Samples were examined using an FEI Tecnai G2 Spirit transmission electron microscope (FEI Company, Hillsboro, Oregon, USA) operating at 80-kV accelerating voltage. Images were recorded with a side-mounted Olympus Veleta CCD camera 4k using EMSIS RADIUS software at a nominal magnification of typically 150,000×.

Aliquots of 5 μl sample were adsorbed onto a glow-discharged carbon film-coated copper grid, washed with three droplets of pure water and subsequently stained with 2% uranyl-acetate. Images were recorded using a Philips CM10 TEM (The Netherlands) operating at 80 kV on a Veleta 4 k CCD camera (Olympus, Germany).
Reference-free alignment was performed on manually selected particles from electron micrographs using the EMAN image-processing package [51 (link)]. Next, particle projections were classified by multi-variant statistical analysis. The class averages with the best signal-to-noise ratio were selected and gathered in a gallery.