Apoptotic cells were identified by the DeadEnd Fluorometric TUNEL System (Promega, Madison, WI, USA) on frozen heart sections according to the manufacturer's protocol.53 (link) Co-staining of TUNEL and anti-Gr1 (1 : 100), anti-F4/80 (1 : 100) (all from Abcam, Cambridge, MA, USA) and anti-α-actinin (1 : 800; Sigma Aldrich, St Louis, MO, USA) was performed to further clarify the cell type of apoptotic cells. Images were captured by a Leica TSC-SP5 laser-scanning confocal microscope (Leica, Wetzlar, Germany).
Anti gr 1
Manufactured by Abcam
Sourced in United States
Anti-Gr-1 is a lab equipment product that detects the Gr-1 antigen, which is expressed on the surface of granulocytes, including neutrophils, eosinophils, and myeloid-derived suppressor cells. This product can be used for the identification and enumeration of these cell populations in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Anti α actinin, by Merck Group (1 mentions)
Deadend fluorometric tunel system, by Promega (1 mentions)
Anti f4 80, by Abcam (1 mentions)
Anti phospho erk, by Abcam (1 mentions)
Alexa fluor 488 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Anti phospho erk, by Cell Signaling Technology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
3 3 diaminobenzidine (dab), by Dojindo Laboratories (1 mentions)
Anti gr 1 rb6 8c5, by BioLegend (1 mentions)
Anti ki67, by Abcam (1 mentions)
Anti cd11b, by Cell Signaling Technology (1 mentions)
Alexa fluor 488 goat anti rat igg, by Cell Signaling Technology (1 mentions)
Cxcr4, by Proteintech (1 mentions)
4 protocols using anti gr 1
1
Detecting Apoptotic Cells in Cardiac Tissue
2
Immunohistochemical Analysis of Mouse Lung
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Mouse lungs were fixed by intratracheal administration of 4% paraformaldehyde in PBS. Paraffin‐embedded tissues were sectioned with 3‐μm thickness and stained with anti‐phospho‐ERK (#4370; Cell Signaling Technology, Danvers, MA, USA), anti‐Gr‐1 (RB6‐8C5; BioLegend), and anti‐OPN (IBL, Fujioka, Gunma, Japan) antibodies. Antibodies were visualized with 3,3′‐diaminobenzidine (Dojindo, Kumamoto, Japan). Frozen sections with 6‐μm thickness were stained with anti‐phospho‐ERK, anti‐Gr‐1, and anti‐citrullinated histone H3 (ab5103; Abcam) antibodies and visualized with Alexa Fluor 488‐conjugated goat anti‐rabbit IgG (Thermo Fisher Scientific). Cell nuclei were stained with DAPI (Sigma‐Aldrich).
3
Histopathological and Immunofluorescence Analysis of Mouse Skin
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As for histopathological analysis, the skin tissues of mice were fixed with 4% paraformaldehyde (PFA) and embedded in paraffin, then cut into sections with a thickness of 5 μm. The skin sections were stained with hematoxylin and eosin (H&E) and visualized with an inverted microscope.
As for immunofluorescence analysis, the skin tissues of mice were fixed with 4% PFA and embedded in OCT, then cut into sections with a thickness of 10 μm. The skin sections were incubated with the following primary antibodies: anti-Ki67 and anti-Gr-1 (Abcam, Cambridge). After washing with PBS, the skin sections were incubated with corresponding conjugated secondary antibodies. The slides were then visualized using an inverted fluorescence microscope. For qualification analysis, at least six representative sections for each group were counted. At least three mice were used in each group. Image J was used for image analysis.
As for immunofluorescence analysis, the skin tissues of mice were fixed with 4% PFA and embedded in OCT, then cut into sections with a thickness of 10 μm. The skin sections were incubated with the following primary antibodies: anti-Ki67 and anti-Gr-1 (Abcam, Cambridge). After washing with PBS, the skin sections were incubated with corresponding conjugated secondary antibodies. The slides were then visualized using an inverted fluorescence microscope. For qualification analysis, at least six representative sections for each group were counted. At least three mice were used in each group. Image J was used for image analysis.
4
Tumor Immunohistochemistry for Invasion and Migration
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Tumor and spleen tissue sections were fixed with ice-cold 4% paraformaldehyde for 15 minutes and treated with 0.2% Triton X-100 for 10 minutes at 37°C. Tissues were blocked with 5% bovine serum albumin for 30 minutes. Then the samples were incubated with different primary antibodies, including anti- matrix metalloproteinase 9 (MMP9; 1:200, cat. no., 10375-2-AP; Proteintech), anti-C-X-C chemokine receptor type 4 (CXCR4; 1:300, cat. no. ab181020; Abcam, Cambridge, MA, USA), anti-Gr-1 (1:100, cat. no. 31469; Cell Signaling Technology, Inc.) and anti-CD11b (1:100, cat. no. 17800, Cell Signaling Technology, Inc.). Then the samples were stained with secondary antibodies for one hour at 37°C (Alexa Fluor 555 anti-rabbit IgG, 1:500, cat. no. 4413, Cell Signaling Technology, Inc.; Alexa Fluor 488 goat anti-Rat IgG, 1:500, cat. no. A-11006; Invitrogen). MMP9 is a marker of tumor invasiveness, and CXCR4 (a chemokine receptor marker for migration) labels the tumor progression. MDSCs are labeled by Gr-1 and CD11b.
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