153Gd(III) chloride and 233U(VI) nitrate were purchased from Eckert & Ziegler Isotope Products (Valencia, CA). 177Lu(III) chloride was purchased from Perkin Elmer Health Sciences (Shelton, CT). A Pu(IV) chloride stock solution containing a 50/50 mixture (Bq/Bq) of 241Pu and 242Pu and a stock solution of 243Am(III), prepared by dissolution of 243Am2O3 in 1 M HNO3, were from LBNL inventory. 225Ac(III), 249Bk(III), and 249Cf(III) were obtained as chlorides from ORNL. A 253Es(III) perchlorate solid sample was provided by Prof. J. Shafer (Colorado School of Mines). 343HOPO was from LBNL inventory14 (link). Standard solutions of 0.1 M and 6.0 M HNO3 (BDH VWR Analytical), 70% HNO3 (Sigma Aldrich), NaNO3 (>99%, ACS grade, VWR), sodium lactate (Sigma Aldrich), TODGA (>99%, Technocomm Ltd.), HDEHP (>95%, Merck), tributyl phosphate (TBP, >98%, Alpha Aesar), kerosene low odor (Alpha Aesar), DTPA (>98%, TCI), and Ultima Gold LLT (Perkin Elmer) were used as received. All solutions were prepared using deionized water purified by a Millipore Milli-Q reverse osmosis cartridge system. Stocks were stored at 8 °C in the dark between experiments.
Milliq reverse osmosis cartridge system
Manufactured by Merck Group
The MilliQ reverse-osmosis cartridge system is a laboratory equipment product that produces high-purity water through a multi-stage purification process. It utilizes reverse osmosis technology to remove impurities and contaminants from water, resulting in purified water suitable for various laboratory applications.
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3 protocols using milliq reverse osmosis cartridge system
1
Radioactive Reagent Characterization Protocol
2
Synthesis and Characterization of Ni(II) Complexes
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The synthesis of L 3S was performed as previously published. 51 Solutions preparation: For the preparation of all the aqueous solutions, ultrapure laboratory grade water that has been filtered and purified by reverse osmosis using Millipore MilliQ reverse-osmosis cartridge system (resistivity 18MΩ cm) (Millipore) was used.
In order to prevent the oxidation of the cysteine residues, all the solutions were prepared under an argon atmosphere in a glovebox. Solutions of ligands or complexes were freshly prepared before each experiment in the appropriate deoxygenated buffer.
Ni II solutions were prepared from the sulfate corresponding salt dissolved in water.
The ligand concentration for the different solutions was determined by the Ellman's procedure. 76 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) is used as an indicator. Each free thiol group present in the ligand yields 1 equiv. of TNB 2-[λ = 412 nm, ε(TNB 2-) = 14150 M -1 cm -1 ].
For the complex solutions, 0.9 equiv. of Ni II were added to the ligand in the proper buffer so that only the mononuclear complex is formed.
In order to prevent the oxidation of the cysteine residues, all the solutions were prepared under an argon atmosphere in a glovebox. Solutions of ligands or complexes were freshly prepared before each experiment in the appropriate deoxygenated buffer.
Ni II solutions were prepared from the sulfate corresponding salt dissolved in water.
The ligand concentration for the different solutions was determined by the Ellman's procedure. 76 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) is used as an indicator. Each free thiol group present in the ligand yields 1 equiv. of TNB 2-[λ = 412 nm, ε(TNB 2-) = 14150 M -1 cm -1 ].
For the complex solutions, 0.9 equiv. of Ni II were added to the ligand in the proper buffer so that only the mononuclear complex is formed.
3
Synthesis and Characterization of P3C and P3L
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Chemicals were purchased from Aldrich, Acros and Fluka and used without further purification.
Enriched 199 HgO (91%) was purchased from Eurisotop. All solutions in water were prepared in ultrapure laboratory grade water that has been filtered and purified by reverse osmosis using Millipore MilliQ reverse-osmosis cartridge system (resistivity 18 Mcm).
The synthesis of P 3C and P 3L was performed as previously published. 17, 18 P 3C was obtained through the linear protected peptide H-Cys(Trt)-Ser(tBu)-Arg(Pbf)-Pro-Gly-Cys(Trt)-Thr(tBu)-Cys(Trt)-Ser(tBu)-Gly-OH. The characterization of the two peptides is reported in the supporting information (SI).
Enriched 199 HgO (91%) was purchased from Eurisotop. All solutions in water were prepared in ultrapure laboratory grade water that has been filtered and purified by reverse osmosis using Millipore MilliQ reverse-osmosis cartridge system (resistivity 18 Mcm).
The synthesis of P 3C and P 3L was performed as previously published. 17, 18 P 3C was obtained through the linear protected peptide H-Cys(Trt)-Ser(tBu)-Arg(Pbf)-Pro-Gly-Cys(Trt)-Thr(tBu)-Cys(Trt)-Ser(tBu)-Gly-OH. The characterization of the two peptides is reported in the supporting information (SI).
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