C5a human elisa duo kit

Manufactured by R&D Systems

The C5a human ELISA duo kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of C5a levels in human serum, plasma, and cell culture supernatants. The kit includes a microplate pre-coated with a monoclonal antibody specific for C5a, and a detection antibody conjugated to horseradish peroxidase (HRP) for colorimetric detection.

Automatically generated - may contain errors

Lab products found in correlation

L lactide, by Polysciences (2 mentions) Human complement c3 elisa kit, by Abcam (1 mentions) Dulbecco s phosphate buffered saline, by Thermo Fisher Scientific (1 mentions) Zymosan, by Merck Group (1 mentions) Proteome profiler human cytokine array kit, by R&D Systems (1 mentions) Rat il 6 quantikine elisa kit, by R&D Systems (1 mentions) 1 3 bis 2 4 6 trimethylphenyl imidazole 2 ylidene imes, by Merck Group (1 mentions)

Spelling variants of this product

ELISAs (144 citations) C5a ELISA (2 citations) Human C5a (2 citations) Human kits (2 citations)

2 protocols using c5a human elisa duo kit

Validating Complement Activation Assay for Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Assay conditions were developed using heparinized human whole blood, plasma, and complement protected human serum, and heparinized porcine whole blood and plasma obtained from Innovative research Inc (Novi, MI). Porcine citrated plasma and human citrated plasma were also obtained from Innovative research Inc (Novi, MI). Zymosan was obtained from Sigma Aldrich. Dulbecco’s phosphate-buffered saline (without phosphate and magnesium) was obtained from Fisher Scientific. C5a human ELISA duo kit (DY2037) was obtained from R&D systems (Minneapolis, MN). Human Complement C3 ELISA Kit (ab108823) and Guinea Pig Complement C3 ELISA Kit (ab157705) were obtained from Abcam plc (Cambridge, MA).
Nanoparticles prepared for validating the assay involved l-lactide from Polysciences Inc (Warrington, PA), heterobifunctional poly(ethylene glycol) with 5000 Da molecular weight from Laysan Biosciences (Arab, AL), and d-lactide from Purac Biomaterials (Corbion, Amsterdam, Netherlands). All solvents used were ACS grade and obtained from Fisher Scientific.

Maisha N., Coombs T, & Lavik E. (2020). Development of a Sensitive Assay to Screen Nanoparticles in vitro for Complement Activation. ACS biomaterials science & engineering, 6(9), 4903-4915.

+ Open protocol

+ Expand

Synthesis of PLA-PEG Block Copolymer

Check if the same lab product or an alternative is used in the 5 most similar protocols

Poly(lactic acid)-b-poly(ethylene glycol) block copolymer was synthesized using L-lactide from Polysciences Inc. (Warrington, PA), heterobifunctional poly(ethylene glycol) with 5000 Da and 3400Da molecular weight from Laysan Biosciences (Arab, AL), and D-lactide from Purac Biomaterials (Corbion, Amsterdam, Netherlands). 1,3-Bis-(2,4,6-trimethylphenyl)imidazole-2ylidene) (iMES) obtained from Sigma Aldrich was used as a catalyst for the ring-opening polymerization. All solvents used were of ACS grade and obtained from Fisher Scientific. In vitro assays used for quantifying C5a, cytokine profile and IL-6.
We monitored Complement protein C5a using a C5a human ELISA duo kit (DY2037) obtained from R&D Systems (Minneapolis, MN). We also measured cytokine levels in vitro using Proteome Profiler Human Cytokine Array Kit (R and D Systems, catalog number ARY005B). For assessing changes in IL-6 in rat endothelial cells, we used the Rat IL-6 Quantikine ELISA Kit (R&D systems, R6000B).

Maisha N., Naik N., Okesola M., Coombs T., Zilberberg R., Pandala N, & Lavik E. (2021). Engineering PEGylated Polyester Nanoparticles to Reduce Complement-Mediated Infusion Reaction. Bioconjugate chemistry, 32(10).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!