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Cxcl 10 and il 6

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CXCL‐10 is a chemokine that regulates immune responses by chemoattracting activated T cells. IL-6 is a cytokine that regulates immune responses and inflammation.

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Lab products found in correlation

Human ifn γ duoset elisa kit, by R&D Systems (1 mentions) Mouse ifn beta duoset elisa kit, by R&D Systems (1 mentions) Cxcl8, by R&D Systems (1 mentions) Sector imager 6000, by Mesoscale (1 mentions) Conventional elisa kits for ifn γ, by BioLegend (1 mentions) Tnf α and ccl2, by Thermo Fisher Scientific (1 mentions)

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IL-10 (345 citations)

2 protocols using cxcl 10 and il 6

1

Cytokine Quantification in T-Cell Derived Extracellular Vesicles

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Protein levels of the cytokines CXCL‐10 and IL‐6 in the cell culture supernatants were determined using the following ELISA kits: CXCL‐10 and IL‐6 (R&D System) according to manufacturer's instructions. IFN‐beta in the cell culture supernatants was determined using the mouse IFN‐beta DuoSet ELISA kit (R&D System) according to manufacturer's instructions. IFNγ in the T‐EVs were determined using the Human IFNγ DuoSet ELISA kit (R&D System) according to manufacturer's instruction. Prior to analysis, the T‐EVs were lyzed in v/v 2.5% Triton‐X‐100, 0.05% Tween‐20.
Hansen A.S., Jensen L.S., Gammelgaard K.R., Ryttersgaard K.G., Krapp C., Just J., Jønsson K.L., Jensen P.B., Boesen T., Johannsen M., Etzerodt A., Deleuran B.W, & Jakobsen M.R. (2023). T‐cell derived extracellular vesicles prime macrophages for improved STING based cancer immunotherapy. Journal of Extracellular Vesicles, 12(8), 12350.
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2

Quantifying Inflammatory Mediators

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Cell-free peritoneal effluents were analyzed on a SECTOR Imager 6000 (Meso Scale Discovery) for IFN-γ, TNF-α, IL-1β, CCL3, CCL4 and CXCL8. Conventional ELISA kits and a Dynex MRX II reader were used for CCL2 (eBioscience) and CCL20 (R&D Systems). Cell culture supernatants were analyzed using conventional ELISA kits for IFN-γ (Biolegend), TNF-α and CCL2 (eBioscience) as well as for CXCL8, CXCL10 and IL-6 (R&D Systems).
Liuzzi A.R., Kift-Morgan A., Lopez-Anton M., Friberg I.M., Zhang J., Brook A.C., Roberts G.W., Donovan K.L., Colmont C.S., Toleman M.A., Bowen T., Johnson D.W., Topley N., Moser B., Fraser D.J, & Eberl M. (2016). Unconventional Human T Cells Accumulate at the Site of Infection in Response to Microbial Ligands and Induce Local Tissue Remodeling. The Journal of Immunology Author Choice, 197(6), 2195-2207.
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