Absorption spectra from the freeze-dried samples were obtained by Fourier transform infrared spectroscopy (FTIR) (Perkin Elmer; Boston, MA, USA) equipped with attenuated total reflectance (ATR). This technique was based on the methodology described by Rahimi and M. Mikani with some modifications [22 (link)]. Samples were placed in intimate contact with the diamond crystal by applying a loading pressure and scanned in a range of 400 cm−1 to 4000 cm−1 at room temperature. The results were analyzed with Spectrum TM 10 software (Perkin Elmer, Boston, MA, USA).
Spectrumtm 10
Manufactured by PerkinElmer
Sourced in United States
The Spectrum™ 10 software is a comprehensive data analysis and processing platform designed for use with various PerkinElmer analytical instruments. It provides a user-friendly interface for data acquisition, processing, and reporting.
Automatically generated - may contain errors
6 protocols using spectrumtm 10
1
FTIR Spectroscopy of Freeze-Dried Samples
2
FTIR Spectroscopy of Samples
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Fourier transform-infrared spectroscopy (FTIR) (Perkin Elmer; Boston, MA, USA) equipment was used to obtain absorption spectra within the range of 380 to 4000 cm−1 wavelengths at room temperature. Samples were placed in intimate contact with the diamond crystal by applying a loading pressure. Four scans with an average of 4 cm−1 resolution were represented in each sample. Automatic signals were collected in 3620 scans with a resolution of 1 cm−1. The spectrum of an empty cell was used as a background. Results were analyzed with SpectrumTM 10 software (Perkin Elmer, Boston, MA, USA).
3
FTIR Analysis of L-Arginine Polymer
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The FTIR spectra of l -Arginine-based polymer and the PMZ/Arg-PA NCs were obtained using a PerkinElmer FTIR spectrometer (Akron, OH, USA). The FTIR spectra were further analyzed using SpectrumTM 10 software obtained from PerkinElmer (OH, USA). A few milligrams were positioned on the main lens, the FTIR scans were recorded over the range of 500–4000 cm−1, and the resolution was set at 2 cm−1.
4
ATR-FTIR Spectroscopic Analysis Protocol
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Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) was conducted using a Perkin Elmer Spectrum One FT-IR spectrometer (C-001), Waltham, MA, United States fitted with a universal ATR sampling accessory. All data were recorded at room temperature at approximately 22 °C, in the spectral range of 4000–650 cm−1, utilising a fixed universal compression load of 75 N and a 4-scan per sample cycle. Subsequent analysis was performed using Perkin Elmer spectrum software (SpectrumTM 10).
5
Efficient Green FTIR Quantification Protocol
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The FTIR technique offers a green alternative because it allows us to quantify substances without organic solvents. The samples do not require any pretreatment, thus reducing the environmental damage caused by toxic waste. Also, it is a fast technique based on the natural vibrational frequencies of the chemical bonds present in molecules. FTIR is a non-destructive technique using a minimum amount of sample [72 (link)]. The absorption spectra by the FTIR technique were obtained with the Frontier FT-MIR (Perkin Elmer. MA, USA) equipment. The wavelength ranged from 380 to 4000 cm−1 at room temperature. The samples were brought into intimate contact with the diamond crystal by applying a loading pressure. For each sample, the spectrum represented an average of four scans with 4 cm−1 resolution. A spectrum of the empty cell was used as the background. Automatic signals were collected in 3620 scans at 1 cm−1 resolution. All the data were processed with SpectrumTM 10 (Perkin Elmer. MA, USA) software.
6
FTIR and NMR Spectroscopic Analysis of Scaffold
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FTIR is a non-destructive technique using a minimum amount of sample.35 The absorption spectra by the FTIR technique were obtained with the spectrum-GX (PerkinElmer) equipment. The wavelength ranged from 400 to 4000 cm−1 at room temperature. The samples were brought into intimate contact with the KBr by applying a loading pressure in reflectance mode. All the data were processed with Spectrum TM 10 (PerkinElmer) software and baseline correction was done.
In NMR spectra analysis, the 400 MHz IH-NMR experiments were performed on a Bruker AM-400 MHz device. Deuterated DMSO was used as a solvent to dissolve C2M1N scaffold and mucin protein at 10 mg mL−1 concentration.36 (link)
In NMR spectra analysis, the 400 MHz IH-NMR experiments were performed on a Bruker AM-400 MHz device. Deuterated DMSO was used as a solvent to dissolve C2M1N scaffold and mucin protein at 10 mg mL−1 concentration.36 (link)
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