Ultra-pure water (18 mΩ) was obtained from a Milli-Q water purification system (Millipore Co., Ltd., Milford, MA, USA). High-performance liquid chromatography (HPLC)-grade methanol, acetic acid, and 56 analytical standards, including catechinic, scopolin, chlorogenic acid, epicatechinic, vanillic acid, caffeic acid, purerarin, syringic acid, daidzin, glycitin, scopoletin, eriocitrin, umbelliferone, p-coumaric acid, dihydroquercetin, sinapic acid, genistin, liquiritin, ferulic acid, salicylic acid, rutin, isoferulic acid, m-coumaric acid, naringin, hesperidin, resveratrol, xanthotoxol, silydianin, sinapyl alcohol, o-coumaric acid, liquiritigenin, kaempferol, 2’-hydroxygenistein, eriodictyol, daidzein, psoralen, glycitein, quercetin, didymin, bergaptol, naringenin, luteolin, cinnamic_acid, hesperetin, genistein, bergapten, diosmetin, isoliquiritigenin, coumestrol, sinensetin, formononetin, medicarpin, imperatorin, biochanin A, tangeretin, and rotenone (displayed in Table S2 ), were purchased from Sigma-Aldrich Co., Ltd. The stock solutions of these authentic standards were 10.0 mg of each standard dissolved in 10 mL methanol. Then, the stock solutions were diluted to various concentrations before analysis. All stock solutions were sealed with Parafilm® and stored in a −20 °C freezer.
Medicarpin
Manufactured by Merck Group
Sourced in United States
Medicarpin is a laboratory equipment product. It is a precision instrument used for various analytical and scientific applications. The core function of Medicarpin is to facilitate accurate measurements and data collection. No further details are available in an unbiased and factual manner.
Automatically generated - may contain errors
Lab products found in correlation
Daidzein, by Merck Group (3 mentions)
Genistein, by Merck Group (3 mentions)
Salicylic acid, by Merck Group (3 mentions)
Naringenin, by Merck Group (3 mentions)
Formononetin, by Merck Group (3 mentions)
Liquiritigenin, by Merck Group (3 mentions)
Biochanin a, by Merck Group (3 mentions)
Scopoletin, by Merck Group (2 mentions)
Jasmonic acid, by Merck Group (2 mentions)
2 hydroxyformononetin, by Merck Group (2 mentions)
2 methoxyformononetin, by Merck Group (2 mentions)
Lc ms grade acetonitrile, by Avantor (2 mentions)
Rotenone, by Merck Group (1 mentions)
Umbelliferone, by Merck Group (1 mentions)
Tangeretin, by Merck Group (1 mentions)
Eriodictyol, by Merck Group (1 mentions)
Eriocitrin, by Merck Group (1 mentions)
Hesperidin, by Merck Group (1 mentions)
Sinensetin, by Merck Group (1 mentions)
Naringin, by Merck Group (1 mentions)
4 protocols using medicarpin
1
Analytical Standards for Chromatographic Analysis
2
Comprehensive Phytochemical Analysis Protocol
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All chemicals and reagents used in this study were of ultrapure mass spectrometry grade. LCMS grade acetonitrile, water and methanol were procured from J.T baker (U.S.A) for mobile phase and sample preparation. Analytical grade formic acid from Sigma Aldrich (U.S.A) was used in the mobile phase. Reference standards for the flavonoids (Naringenin, Liquiritigenin, Genistein, Daidzein, Formononetin and Biochanin A) and phytohormones (Salicylic acid and Jasmonic acid) were procured from Sigma-Aldrich, USA.The flavonoids 2'-hydroxyFormononetin, 2'methoxyFormononetin and Medicarpin were synthesized and purified in >98 % purities as described in the literature [32] (link).
3
Phytoalexin Quantification by HPLC
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Phytoalexins were identified and quantified by HPLC using an HPLC (Perkin Elmer, Series 200 System, Monza, Italy) equipped with a diode array detector set to 210 and 220 nm.
Analyses were carried out in duplicate injecting 20 μL of all samples for each analysis.
Standards (scopoletin, pisatin, medicarpin, maackiain (Sigma-Aldrich, St. Luis, MO, USA)) were prepared at concentration of 1 mg ml -1 in methanol and diluted in a 1:10 ratio in a C 18 column 250×4 mm internal diam., containing 5 mm diam. beads (LiChrospher 100 RP-18), using a flow rate of 1 ml min -1 and elution with CH 3 CN/H 2 O along a linear gradient of CH 3 CN from 20 to 33% over a period of 90 min, and again from 33 to 20% for 5 min. The column was re-equilibrated under isocratic conditions for 5 min before the next run. The data obtained were expressed in µg g -1 FW.
Analyses were carried out in duplicate injecting 20 μL of all samples for each analysis.
Standards (scopoletin, pisatin, medicarpin, maackiain (Sigma-Aldrich, St. Luis, MO, USA)) were prepared at concentration of 1 mg ml -1 in methanol and diluted in a 1:10 ratio in a C 18 column 250×4 mm internal diam., containing 5 mm diam. beads (LiChrospher 100 RP-18), using a flow rate of 1 ml min -1 and elution with CH 3 CN/H 2 O along a linear gradient of CH 3 CN from 20 to 33% over a period of 90 min, and again from 33 to 20% for 5 min. The column was re-equilibrated under isocratic conditions for 5 min before the next run. The data obtained were expressed in µg g -1 FW.
4
Comprehensive Phytochemical Analysis Protocol
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All chemicals and reagents used in this study were of ultrapure mass spectrometry grade. LCMS grade acetonitrile, water and methanol were procured from J.T baker (U.S.A) for mobile phase and sample preparation. Analytical grade formic acid from Sigma Aldrich (U.S.A) was used in the mobile phase. Reference standards for the flavonoids (Naringenin, Liquiritigenin, Genistein, Daidzein, Formononetin and Biochanin A) and phytohormones (Salicylic acid and Jasmonic acid) were procured from Sigma-Aldrich, USA.The flavonoids 2'-hydroxyFormononetin, 2'methoxyFormononetin and Medicarpin were synthesized and purified in >98 % purities as described in the literature [32] (link).
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