For ISH detection, deparaffinized tissue sections were hybridized with 50 nM LNA-modified and digoxin-labeled miR-361-5p oligonucleotide probe (Exiqon, Vedbaek, Denmark) for 1 h at 55°C, incubated with 5 μg/mL anti-digoxin-Rhodamine antibody (Roche Applied Science, Penzberg, Germany) overnight at 4°C, and stained with DAPI in the dark for 15 min at RT. The red fluorescence in the cytoplasm indicated positive signal. The IHC staining was performed according to the standard ABC protocol with the antibodies of mouse anti-human SND1 (1:100; Abcam, Cambridge, UK). The brown signal in the cytoplasm was considered to indicate positive result. The labeling index (LI) was calculated as the percentage of positive cell number to the total cell number.
Anti digoxin rhodamine antibody
Manufactured by Roche
Sourced in Germany, United States
The Anti-digoxin-Rhodamine antibody is a laboratory reagent used for the detection and quantification of the cardiac glycoside digoxin in biological samples. This antibody is labeled with the fluorescent dye rhodamine, which allows for the visualization and measurement of digoxin levels using techniques such as immunoassays.
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Lab products found in correlation
2 protocols using anti digoxin rhodamine antibody
1
In Situ Hybridization and IHC for miR-361-5p and SND1
2
In Situ Hybridization and Immunohistochemistry for miRNA and Protein Expression Analysis
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For ISH detection, deparaffinized tissue sections were hybridized with 50 nM LNA-modified and digoxin-labeled miR-320a oligonucleotide probe (Exiqon, Vedbaek, Denmark; Supplementary Table 4 ) for 1 h at 55°C, incubated with 5μg/ml anti-digoxin- Rhodamine antibody (Roche Applied Science, Indianapolis, IN, USA) overnight at 4°C, and stained with DAPI in the dark for 15 min at room temperature (RT). The IHC staining was performed according to the standard ABC protocol with the antibodies of mouse anti-human SND1 (Abcam, Cambridge, MA, USA), rabbit anti-human β-catenin (CST, Boston, MA, USA) and rabbit anti-human Ki-67 (Millipore, Billerica, MA, USA). The labeling index (LI) is presented as the percentage of positive cell number to total cell number.
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