Tie2 cre 0 b6 cg tg tek cre 1ywa j mice

The generation of Igf2 mutant mice has been described previously39 (link). Mice were maintained on a C57BL6 background and crossed with CD1 to generate viable adult offspring of wild-type (Igf2^+/+), maternal (Igf2^mat/+) and paternal (Igf2^+/pat) transmission heterozygotes and homozygous null Igf2^mat/pat mutant mice. Igf2 mutant animals were genotyped by PCR analysis of DNA, extracted from mouse ear-punch tissue with the following primers: Igf2-E6F (5′-TGGCCTGGTATCCAAAACAT-3′), Igf2-E6R (5′-CTGGATGACATGGACAGTGG-3′) and Neo-E6F (5′-AGCGCATCGCCTTCTATC-3′). Tie2-cre^+/0(B6.Cg-Tg (Tek-cre)1Ywa/J) mice were obtained from The Jackson Laboratory. Igf2;Tie2-cre floxed mice were generated as described previously43 (link), and genotypes were determined with the following primers: for the deletion, II-delF (5′-TTACAGTTCAAAGCCACCACG-3′), II-delRW (5′-GCCAAAGAGATGAGAAGCACC-3′) and II-delRD (5′-GCCAAACACAGTAAAAAGAAATGC-3′). For the transgene, oIMR1084F (5′-GCGGTCTGGCAGTAAAAACTATC-3′) and oIMR1085R (5′-GTGAAACAGCATTGCTGTCACTT-3′). Internal control, oIMR7338F (5′-CTAGGCCACAGAATTGAAAGATCT-3′) and oIMR7339R (5′-GTAGGTGGAAATTCTAGCATCATCC-3′). See also ‘Imprinting assay' below. Housing of mice and all experiments were carried out in accordance with UK and Spanish Government Home Office licensing procedures.