Elisa kit specific for mouse il 6

Manufactured by BD

The BD ELISA kit is a quantitative immunoassay for the measurement of mouse Interleukin-6 (IL-6) in various sample types. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte.

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ELISA kits for IL-6 Specific ELISA kits Mouse IL-6 Specific ELISAs Mouse ELISA kits ELISAs

3 protocols using elisa kit specific for mouse il 6

Serum Biomarkers in Mouse Liver Injury

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Whole-blood samples were centrifuged at 4,000 revolutions/min for 12 min to collect serum. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) in the serum were determined by assay kits from Pointe Scientific (Lincoln Park, Mich). Serum and liver tissue IL-6 levels were determine by an enzyme-linked immunosorbent assay (ELISA) kit specific for mouse IL-6 (BD Biosciences, San Diego, Calif). These assays were carried out according to the manufacturer's instructions.

Godwin A., Yang W.L., Sharma A., Khader A., Wang Z., Zhang F., Nicastro J., Coppa G.F, & Wang P. (2015). Blocking Cold-Inducible RNA-Binding Protein (CIRP) Protects Liver from Ischemia/Reperfusion Injury. Shock (Augusta, Ga.), 43(1), 24-30.

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Serum Biomarker Analysis in Mice

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Whole blood samples were centrifuged at 7,000 rpm for 10 min and the serum was aspirated and stored at -80°C. Commercial assay kits from Pointe Scientific (Lincoln Park, MI) were used to measure the activity levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH). Serum IL-6 levels were determined by an enzyme-linked immunosorbent assay (ELISA) kit specific for mouse IL-6 (BD Biosciences, San Diego, CA). The assays were carried out according to the manufacturer’s instructions.

Hansen L.W., Khader A., Yang W.L., Prince J.M., Nicastro J.M., Coppa G.F, & Wang P. (2016). Sirtuin 1 Activator SRT1720 Protects against Organ Injury Induced by Intestinal Ischemia-Reperfusion. Shock (Augusta, Ga.), 45(4), 359-366.

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Serum Injury Biomarker Measurement

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Blood samples were centrifuged at 2,000g for 15 min to collect serum and then either analyzed for injury parameters immediately, or stored at −80°C. Blood urea nitrogen (BUN), and creatinine were measured by using commercial assay kits according to the manufacturer’s instructions (Pointe Scientific; Lincoln Park, MI). IL-6 levels were determine by an enzyme-linked immunosorbent assay (ELISA) kit specific for mouse IL-6 (BD Biosciences; San Diego, CA).

Cen C., Yang W.L., Yen H.T., Nicastro J.M., Coppa G.F, & Wang P. (2016). Deficiency of Cold-inducible RNA-binding Protein Reduces Renal Injury after Ischemia-Reperfusion. Surgery, 160(2), 473-483.

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