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Malondialdehyde mda elisa kit

Manufactured by Nanjing Jiancheng
Sourced in United States

The Malondialdehyde (MDA) ELISA kit is a laboratory equipment used for the quantitative determination of MDA levels in various biological samples. MDA is a biomarker for oxidative stress and lipid peroxidation. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) principle to measure MDA concentrations.

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2 protocols using malondialdehyde mda elisa kit

1

Antioxidant Biomarker Assay Protocol

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The following chemicals were purchased from the suppliers given in brackets: citric acid (Beijing Chemical Reagent Company, batch No.: 060403), ammonia (Sinopharm Chemical Reagent Co., Ltd., batch No.: 20120918), phenol red (Solarbio Company, batch No.: 20114.12.07), nutrient broth (China Pharmaceutical and Biological Products), ether (Beijing Reagent Company, batch No.: 20090707), superoxide dismutase (SOD) ELISA kit (Nanjing Jiancheng Institute of Biotechnology, batch No.: 201209), malondialdehyde (MDA) ELISA kit (Nanjing Jiancheng Institute of Biotechnology, batch No.: 201209), and micro BCA protein assay kit (Kangwei Century Company, batch No.: 201209).
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2

Herbal Neuroprotection Protocol

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The 6 raw herbs of DSS were purchased from Kangmei Pharmaceutical Limited Company (Guangzhou, China). Donepezil hydrochloride (D849374) and scopolamine hydrobromide trihydrate (S860151) were from Macklin (Shanghai, China); FITC-dextran (#46944) was from Sigma system (ChemiDoc MP, Bio-Rad, California, USA); total cholesterol Assay Kit (A111-1-1) and Triglyceride Assay Kit (A110-1-1) were from Nanjing Jiancheng; and Malondialdehyde (MDA) ELISA Kit (JL13339), Adiponectin (ADPN) Kit (JL20696D), Low-Density Lipoprotein Cholesterol (LDL-C) Kit (JL20313), High-Density Lipoprotein Cholesterol (HDL-C) Kit (JL20356), Tumour Necrosis Factor-α Kit (TNF-α) (JL10484) and Interleukin 6 (IL-6) Kit (JL20313) were from Jiang Lai Biological (Shanghai, China). Antibodies included rabbit anti-ZO-1 (PB9234, Boster, Pleasanton, CA, USA), rabbit anti-OCLN (A01246-2, Boster), rabbit anti-OCLN (#91131, CST, Danvers, MA, USA) in brain immunofluorescence, rabbit anti-ZO-1 (#13663, CST) in brain immunofluorescence, rabbit anti-PPAR-γ (#2435, CST), Cy3-labelled Goat Anti-Rabbit IgG (H+L) (A0516, Beyotime, Shanghai, China), rabbit anti-LXR alpha+beta (ab21669, Abcam, Cambridge, MA, USA), rabbit anti-actin (#2118, CST), and rabbit anti-GAPDH (AP0063, Bioworld, St. Louis Park, MN, USA).
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