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Uas tkvca

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The UAS-TkvCA is a laboratory equipment designed for specific experimental purposes. It serves a core function within the context of controlled laboratory environments, but a detailed description without potential interpretation or extrapolation is not available.

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Lab products found in correlation

Nubbin gal4, by BD (1 mentions) Uas ryrrnai, by BD (1 mentions) W1118, by Bloomington Drosophila Stock Center (1 mentions) Uas dicer2, by Vienna Drosophila Resource Center (1 mentions) Uas dicer2, by BD (1 mentions) Uas rab11 gfp, by BD (1 mentions)

2 protocols using uas tkvca

1

Drosophila Genetic Crosses and Stocks

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Drosophila were raised in a 25 °C incubator with a twelve-hour light cycle unless specified otherwise. Virgins from Gal4 lines were collected twice a day from the bottles. Virgins were crossed with males carrying the indicated UAS line constructs in a ratio of females to males 15:5. The crosses were staged for 4 h to collect the correct aged larvae. The wild-type Oregon-R fly line is a long-standing stock in the Zartman lab acquired from the N. Yakoby lab. The following transgenic stocks were obtained from Bloomington Drosophila Stock Center (BDSC): UAS-RyRRNAi (BDSC#31540), UAS-InsRDN(BDSC#8253), UAS-InsRCA(BDSC#8263), UAS-TkvRNAi (BDSC#31041), UAS-TkvCA(BDSC#36537), Nubbin-Gal4 (BDSC#25754), Engrailed-Gal4 (BDSC#25752), UAS-Myc (BDSC#9674), UAS-mTORRNAi (BDSC#33951), UAS-MycRNAi (BDSC#25783), and UAS-mTORDN (BDSC#7013).
Kumar N., Rangel Ambriz J., Tsai K., Mim M.S., Flores-Flores M., Chen W., Zartman J.J, & Alber M. (2024). Balancing competing effects of tissue growth and cytoskeletal regulation during Drosophila wing disc development. Nature Communications, 15, 2477.
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2

Drosophila Strain Characterization and Manipulation

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Several Drosophila strains were used, including: w1118 (Bloomington, USA, BDSC); witA12 (BDSC); C380-GAL4 (BDSC); OK6-GAL4 (BDSC), UAS-dicer2; UAS-dsRNA-atl (Vienna Drosophila Resource Center, Austria, VDRC), UAS-dicer2, UAS-tkv-CA, UAS-rab4-GFP, UAS-rab11-GFP and Rab7-YFP (BDSC). A detailed table with all the stocks used can be found in Additional file 1: Table S1.
Bertin F., Jara-Wilde J., Auer B., Köhler-Solís A., González-Silva C., Thomas U, & Sierralta J. (2023). Drosophila Atlastin regulates synaptic vesicle mobilization independent of bone morphogenetic protein signaling. Biological Research, 56, 49.
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