Irdye 800cw

Commercial antibodies used for Western blotting were the following:
Anti-HSP-4/BiP (Novus Biologicals, #NBP1-06274, 1:1000 dilution)
Anti-RFP (GenScript, #A00682, 1:1000 dilution)
Anti-FLAG (Sigma, #F3165, 1:1000 dilution)
Anti-LMP-1(Developmental Studies Hybridoma Bank, #LMP1, 1:100 dilution)
Anti-HSP-70/HSC-70 (Santa Cruz Biotechnology Inc., #sc-33575, 1:1000 dilution)
Anti-calnexin (Novus Biologicals, #NBP1-97476, 1:1000 dilution)
Anti-CPL-1 (Abcam, #ab58991, 1:500 dilution)
Anti-actin (EMD Millipore, #MAB1501R, 1:5000 dilution)
Goat anti-mouse (LI-COR IRDye 800CW, #925–32210, 1:10,000 dilution)
Goat anti-rabbit (LI-COR IRDye 800CW, #925–32211, 1:10,000 dilution)
Donkey anti-goat (LI-COR IRDye 800CW, #925–32214, 1:10,000 dilution)
Donkey anti-mouse (LI-COR IRDye 680RD, #925–68072, 1:10,000 dilution)
Antibodies made in-house and used for Western blotting were the following:
Anti-granulin 1(RB2481, Biomatik, epitope HQCDAETEC(acm)SDDET, 1:1000 dilution)
Anti-granulin 3 (RB2487, Biomatik, epitope CTVLMVESARSTLKL, 1:1000 dilution)
Anti-ASP-3 (Fred Hutchinson, epitope CTGPTDVIKKIQHKIG, 1:1000 dilution)
Imaging and quantification were performed on the LI-COR Odyssey Infrared System. Three independent blots were performed.

The antibodies used for Western blot were monoclonal Anti-Proliferating Cell Nuclear Antigen (PCNA) (clone PC10 Sigma-Aldrich P8825; 1:3000), monoclonal anti-alpha-actin (Sigma-Aldrich HHF35; 1:5000), monoclonal anti-myosin heavy chain type IIa (DSHB SC-71; 1:1000), monoclonal anti-Flag (Sigma-Aldrich F1804; 1:6000) and polyclonal anti-UBE2L3 (Sigma; 1:1000). The secondary antibodies used were anti-mouse (IRDye^® 800 CW, Licor 92632210 or IRDye^® 680, Licor 92632220;), anti-goat (IRDye^® 800 CW, Licor 92632214) and anti-rabbit (IRDye^® 800 CW, Licor 92632221). The antibody used for skeletal muscle cross-sectional analysis was anti-laminin-α1 (L9393) from Sigma.
Heavy Meromyosin (HMM; Cat. # MH01) (including the first 800 amino acids of MHC plus the two pairs of light chains) and alpha-actin (Catalogue No. AKL99) purified from rabbit skeletal muscle were purchased from Cytoskeleton, Inc., Denver, CO, USA. Alpha-actin was reconstituted in General Actin Buffer (5 mM Tris-HCl pH 8.0, 0.2 mM CaCl2 and 0.2 mM ATP) supplemented with 0.2 mM ATP and 0.5 mM DTT, following the supplier’s instructions. Then 1/10th volume of Polymerization Buffer (500 mM KCl, 20 mM MgCl2, 10 mM ATP) was added to induce the polymerization of a-actin. UBE2G2, UBE2N, UBE2V2, UBE2Z and UBE2K were purchased from LifeSensors. UBE2D2 and UBE2C were purchased from Enzo Life Sciences.

Western Blot: mouse anti-αSyn (Syn1 Clone 42,BD transduction labs, 610,787, 1:500 WB); rabbit anti-pS129 αSyn (MJFR13, Abcam 168,381, 1:500 WB); rabbit anti-vinculin (clone E1E9V, Cell Signaling Technologies 13,901, 1:1000 WB). Secondary Antibodies for Western Blot were as follows: LiCor IRDye 800CW – Dk α Ms (926–32,212) 1:5000; LiCor IRDye 800CW – Donkey anti-Rabbit (926–32,213) 1:5000; LiCor IRDye 680RD – Donkey anti-Mouse (926–68,072) 1:10,000; LiCor IRDye 800RD–Donkey-anti-Rabbit (926–68,073) 1:10,000.
Immunohistochemistry: Rabbit anti-VGLUT2 (Synaptic Systems Cat # 135 403, 1:500 IHC); Chicken anti-MAP2 (Biolegend Cat # 822,501, 1:1000 IHC); Mouse anti-GAD1/67 (Millipore Sigma Cat # MAP5406, 1:500 IHC); Rabbit anti-PAX6 (Invitrogen Cat # 426,600, 1:200 IHC); Mouse anti-S100β (Sigma, Cat # S2532, 1:500 IHC).
Secondary antibodies for IHC were as follows: Hoechst: Hoechst 33,342 Solution (20 mM) Cat #:62,249 (1:1000). Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (ThermoFisher A11034); goat anti-Chicken IgY (H + L) Secondary Antibody, Alexa Fluor 555 (ThermoFisher A21437); goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 (ThermoFisher A21236).