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Human synoviocyte growth medium

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Human synoviocyte growth medium is a specialized cell culture medium designed to support the growth and maintenance of human synoviocytes, which are cells found in the synovial membrane lining the inside of joint cavities. This medium provides the necessary nutrients and growth factors required for the optimal proliferation and survival of these cells in vitro.

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Lab products found in correlation

Rpmi 1640 medium, by American Type Culture Collection (3 mentions) Ovcar 3 cells, by American Type Culture Collection (3 mentions) Hfls ra, by Cell Applications (3 mentions) Jurkat cell, by American Type Culture Collection (1 mentions) Ovcar3, by American Type Culture Collection (1 mentions) Jurkat e6, by American Type Culture Collection (1 mentions)

3 protocols using human synoviocyte growth medium

1

Culturing Human Cell Lines

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Human epithelial ovary adenocarcinoma (OVCAR-3) human T-lymphocyte cells (Jurkat, E6–1) were purchased from American Type Culture Collection (ATCC). Jurkat cells were cultured in RPMI-1640 medium (ATCC) with 10% FBS, 0.01 mg/mL bovine insulin and 0.1 mg/mL penicillin/streptomycin. OVCAR-3 cells were cultured in RPMI-1640 medium from ATCC, with 20% FBS, 0.01 mg/mL bovine insulin, and 0.1 mg/mL penicillin/streptomycin. Human fibroblast-like synoviocytes rheumatoid arthritis (HFLS-RA) cells were purchased from Cell Applications, Inc., San Diego, CA, USA, and maintained in a human synoviocyte growth medium purchased from Cell Applications, Inc. All cell lines were maintained at 37 °C and 5% CO2.
Dahal A., Parajuli P., Singh S.S., Shrestha L., Sonju J.J., Shrestha P., Chatzistamou I, & Jois S. (2022). Targeting protein–protein interaction for immunomodulation: A sunflower trypsin inhibitor analog peptidomimetic suppresses RA progression in CIA model. Journal of pharmacological sciences, 149(3), 124-138.
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2

Culturing Human Cell Lines

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Human epithelial ovary adenocarcinoma (OVCAR-3) human T-lymphocyte cells (Jurkat, E6–1) were purchased from American Type Culture Collection (ATCC). Jurkat cells were cultured in RPMI-1640 medium (ATCC) with 10% FBS, 0.01 mg/mL bovine insulin and 0.1 mg/mL penicillin/streptomycin. OVCAR-3 cells were cultured in RPMI-1640 medium from ATCC, with 20% FBS, 0.01 mg/mL bovine insulin, and 0.1 mg/mL penicillin/streptomycin. Human fibroblast-like synoviocytes rheumatoid arthritis (HFLS-RA) cells were purchased from Cell Applications, Inc., San Diego, CA, USA, and maintained in a human synoviocyte growth medium purchased from Cell Applications, Inc. All cell lines were maintained at 37 °C and 5% CO2.
Dahal A., Parajuli P., Singh S.S., Shrestha L., Sonju J.J., Shrestha P., Chatzistamou I, & Jois S. (2022). Targeting protein–protein interaction for immunomodulation: A sunflower trypsin inhibitor analog peptidomimetic suppresses RA progression in CIA model. Journal of pharmacological sciences, 149(3), 124-138.
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3

Cell Culture Conditions for Cancer and Immune Research

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Human ovarian carcinoma cells (OVCAR-3) and human T-lymphocyte cells (Jurkat, E6-1) were purchased from the American Type Culture Collection (ATCC). OVCAR-3 cells were cultured in RPMI-1640 medium from ATCC, with 20% FBS, 0.01 mg/mL bovine insulin, and 0.1 mg/mL penicillin/streptomycin. Jurkat cells were cultured in RPMI-1640 medium (ATCC) with 10% FBS, 0.01 mg/mL bovine insulin and 0.1 mg/mL penicillin/streptomycin. Human fibroblast-like synoviocytes rheumatoid arthritis (HFLS-RA) cells were purchased from Cell Applications, Inc., San Diego, CA, USA, and maintained in human synoviocyte growth medium purchased from Cell Applications, Inc. OVCAR-3 cells and HFLS-RA cells were used as a model for cells expressing CD58 protein, and Jurkat cells were used as a model for human T cells.
Parajuli P., Sable R., Shrestha L., Dahal A., Gauthier T., Taneja V, & Jois S. (2020). Modulation of costimulatory signal from CD2-CD58 proteins by a grafted peptide. Chemical biology & drug design, 97(3), 607-627.
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