Vs120 microscope

Manufactured by Olympus

Sourced in Japan, Panama

The VS120 is a digital slide scanning microscope designed for high-resolution imaging. It features a high-performance optical system and advanced imaging capabilities to capture detailed images of biological samples. The VS120 is intended for use in research and clinical laboratory settings.

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Lab products found in correlation

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Close spelling variants of this product

VS120 Virtual Slide Microscope System VS120–L100 Virtual Slide Microscope Virtual Slide Microscope (VS120-S6-W, Virtual Slide microscope VS120-SL VS120 Automated Slide Scanning Microscope Microscope VS120 Whole Slide Scanner VS120 slide scanner microscope VS120-L100-W virtual slide microscope VS120 virtual microscope VS120 epifluorescence microscope

80 protocols using vs120 microscope

Quantifying Neuronal Co-localization and MOR Expression

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Images were taken using Olympus VS120 microscope, Olympus FV3000 confocal fluorescence microscope and Nikon Tie-A1 plus confocal fluorescence microscope. For co-localization analysis, confocal images taken by Olympus FV3000 were cropped in 400 μm × 400 μm areas. The number of counting areas ranges from 1 to 6, which depends on the area of each brain region in one section. Cell counting was carried out manually using Fiji (NIH). For the calculating of MOR re-expression efficiency using MOR DAB staining, images were taken by Olympus VS120 microscope and processed in Fiji. Images were transformed to 8-bit and substrated the background, and then gray value in specific brain regions were measured in 800 μm × 800 μm areas, except for the CeA that 600 μm × 600 μm areas were used in Vgat-Cre/Oprm1^KI/KI group. The expression level of MOR was normalized to the mean gray value of MOR signal in each brain region of wild-type mice.

Zhang X.Y., Dou Y.N., Yuan L., Li Q., Zhu Y.J., Wang M, & Sun Y.G. (2020). Different neuronal populations mediate inflammatory pain analgesia by exogenous and endogenous opioids. eLife, 9, e55289.

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Lipid and Nuclei Staining in Cryosections

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Cryosections (5 µm) were dried at room temperature for 2 h. BODIPY™ 493/503 was used to stain neutral lipids (0.25 µg/mL, ThermoFisher Scientific, Cat#D3922). Hoechst 33342 Solution was used to stain nuclei (0.1 µg/mL). Sections were incubated in solution made of 1× PBS with BODIPY and Hochest for 15 min. Sections were washed five times for 3 min in 1× PBS. The whole sections were imaged with a VS120 Olympus microscope. Images were analyzed with Image J.

Murphy K., Zhang A., Bittel A.J, & Chen Y.W. (2023). Molecular and Phenotypic Changes in FLExDUX4 Mice. Journal of Personalized Medicine, 13(7), 1040.

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Multiplexed Immunohistochemistry for SARS-CoV-2

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Tissue sections from paraffin-embedded lung fragments obtained from COVID-19 fatal cases were tested by immunohistochemistry using anti–SARS-CoV-2 polyclonal antibody for in situ detection of SARS-CoV-2. Sequential immunoperoxidase labeling and erasing was then performed to determine additional markers after SARS-CoV-2 immune stain, using antibodies to CD14 (1:100 dilution; Abcam) and NLRP3 (1:100 dilution; Cell Signaling). After the incubation with primary antibody, the slides were incubated with an immunoperoxidase polymer anti-mouse visualization system (SPD-125; Spring Bioscience, Biogen) and then with the chromogen substrate 3-amino-9-ethylcarbazole (AEC) peroxidase system kit (SK-4200; Vector Laboratories). Microphotographs after immunostaining of tissue slides were scanned on a VS120 Olympus microscope. After high-resolution scanning, slides with coverslips were removed in PBS and dehydrated through an ethanol gradient to 95% ethanol. Slides were incubated in ethanol series until AEC color reaction was erased. Following rehydration, antibodies were eluted by incubating sections in 0.15 mM KMnO₄/0.01 M H₂SO₄ solution for 2 min, followed immediately by a distilled water wash. Tissue was then restained as indicated in the blocking step.

Rodrigues T.S., de Sá K.S., Ishimoto A.Y., Becerra A., Oliveira S., Almeida L., Gonçalves A.V., Perucello D.B., Andrade W.A., Castro R., Veras F.P., Toller-Kawahisa J.E., Nascimento D.C., de Lima M.H., Silva C.M., Caetite D.B., Martins R.B., Castro I.A., Pontelli M.C., de Barros F.C., do Amaral N.B., Giannini M.C., Bonjorno L.P., Lopes M.I., Santana R.C., Vilar F.C., Auxiliadora-Martins M., Luppino-Assad R., de Almeida S.C., de Oliveira F.R., Batah S.S., Siyuan L., Benatti M.N., Cunha T.M., Alves-Filho J.C., Cunha F.Q., Cunha L.D., Frantz F.G., Kohlsdorf T., Fabro A.T., Arruda E., de Oliveira R.D., Louzada-Junior P, & Zamboni D.S. (2020). Inflammasomes are activated in response to SARS-CoV-2 infection and are associated with COVID-19 severity in patients. The Journal of Experimental Medicine, 218(3), e20201707.

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Haematoxylin-Eosin Tissue Structure Analysis

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Haematoxylin-eosin staining was applied to observe tissue structure. Cryosections (8 μm thickness) were fixed with ethanol (20 min), washed with distilled water (3 times, 5 min each) and treated with Weigert’s haematoxylin (20 min) for nuclei staining. Then, the cryosections were washed with running tap water (20 min), followed by distilled water (3 times, 5 min each). The cytoplasm was counterstained with 1% eosin alcoholic solution (1 min). After washing with distilled water (3 times, 5 min each), the stained sections were embedded in glycerine jelly. Whole cryosections were scanned by a VS120 Olympus microscope with OlyVIA software (Olympus, Japan). GIMP software was used to identify four histologically different zones of tissue in the scanned pictures. Three to six rectangular areas of each zone per cryosection were chosen for comparison with Zn and Cu maps to detect their local contents.

Anyz J., Vyslouzilova L., Vaculovic T., Tvrdonova M., Kanicky V., Haase H., Horak V., Stepankova O., Heger Z, & Adam V. (2017). Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration. Scientific Reports, 7, 40169.

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Histological Analysis of Osteosarcoma

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At the animal experimental end point, the osteosarcoma tumors and main organs were collected for further analysis. After fixation, the tissues were embedded in paraffin, sliced into sections and histologically examined following hematoxylin and eosin (H&E) staining. The images were acquired using a VS120 Olympus microscope with OlyVIA software (Olympus).

Wang G., Cui Z., Tian J., Li X., Tang W., Jing W., Li A, & Zhang Y. (2024). Paucatalinone A from Paulownia Catalpifolia Gong Tong Elicits mitochondrial-mediated cancer cell death to combat osteosarcoma. Frontiers in Pharmacology, 15, 1367316.

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Immunohistochemical Analysis of CPS1

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The samples were sectioned, deparaffinized, dehydrated and boiled in citrate buffer. Then immunohistochemical staining was performed using human polyclonal antibodies against CPS1. All images were acquired using a VS120 Olympus microscope (Olympus).

Jing W., Chen C., Wang G., Han M., Chen S., Jiang X., Shi C., Sun P., Yang Z., Shi B, & Jiang X. (2023). Metabolic Modulation of Intracellular Ammonia via Intravesical Instillation of Nanoporter‐Encased Hydrogel Eradicates Bladder Carcinoma. Advanced Science, 10(12), 2206893.

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Hematoxylin and Eosin Staining Protocol

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For hematoxylin and eosin staining, the mice were sacrificed at the endpoint, and the tumor tissue was carefully removed. After fixation with 4% paraformaldehyde, the tissue was embedded and stained using a standard protocol. Images were acquired using a VS120 Olympus microscope with OlyVIA software (Olympus).

Jing W., Wang G., Cui Z., Xiong G., Jiang X., Li Y., Li W., Han B., Chen S, & Shi B. (2022). FGFR3 Destabilizes PD-L1 via NEDD4 to Control T-cell-Mediated Bladder Cancer Immune Surveillance. Cancer research, 82(1).

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Histological Analysis of Mouse Brains

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Anaesthetized mice (wt, n = 2; hyh n = 2) were transcardially perfused with Bouin´s fixative solution. Then, the brains were postfixed for 72 h and embedded in paraffin to obtain serial frontal sections (10 μm thick). Deparaffinated sections were stained with haematoxylin–eosin. The stained sections were scanned using a VS120 microscope (Olympus, Tokyo, Japan) with a UPLSAPO20x/0.75 objective.

Ojeda-Pérez B., Campos-Sandoval J.A., García-Bonilla M., Cárdenas-García C., Páez-González P, & Jiménez A.J. (2021). Identification of key molecular biomarkers involved in reactive and neurodegenerative processes present in inherited congenital hydrocephalus. Fluids and Barriers of the CNS, 18, 30.

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Fibroblast Karyotype Analysis Protocol

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The karyotype analysis was conducted following a previously described method with minor modifications^{9 (link)}. Fibroblasts were initially treated with Colchicine (100 ng/ml, APExBio, A3324) for 12 h. After washing, the cells were dissociated using 0.25% Trypsin-EDTA and exposed to a pre-warmed hypotonic solution for 30 min at 37 °C. Subsequently, 2–3 ml pre-chilled fixation solution was gently added to the cell and hypotonic solution mixture, and the cells were pre-fixed at room temperature for 10 min. The cells underwent centrifugation and were resuspended with fresh pre-chilled fixation solution three times for proper fixation. Finally, the cells were dropped onto slides and stained with Giemsa (Sigma, GS500). The resulting slides were then observed and imaged using VS120 microscope (Olympus).

Liao Z., Zhang J., Sun S., Li Y., Xu Y., Li C., Cao J., Nie Y., Niu Z., Liu J., Lu F., Liu Z, & Sun Q. (2024). Reprogramming mechanism dissection and trophoblast replacement application in monkey somatic cell nuclear transfer. Nature Communications, 15, 5.

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Histomorphological Analysis of Back Tissue

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Mice were deeply anesthetized with isoflurane and transcardially perfused with 10% Neutral buffered formalin (NBF). Back tissue was post-fixed for 7 days prior to processing and sectioning. The standard protocols for H&E staining were followed. Four micron-thick issue sections adhered to slides were dewaxed and cleared with xylene, hydrated through incubation in a series of decreasing concentrations of alcohols (100 – 70%), stained with filtered hematoxylin, treated with an alkaline solution, and counterstained with eosin. Subsequently, sections were dehydrated in several changes of alcohol, cleared, and coverslipped. Sections were imaged using an Olympus VS120 microscope (Olympus Corporation, Tokyo, Japan) in brightfield mode. Colored bright-field images of back tissue sections were automatically acquired and stitched by using an automated slide scanner and a 20x objective. Analysis was carried out in FIJI^{43 (link),45 (link)} using line and area measurement. The same analysis parameters were applied across all regions of interest.

Yang Q., Wei T., Yin R.T., Wu M., Xu Y., Koo J., Choi Y.S., Xie Z., Chen S.W., Kandela I., Yao S., Deng Y., Avila R., Liu T.L., Bai W., Yang Y., Han M., Zhang Q., Haney C.R., Lee K.B., Aras K., Wang T., Seo M.H., Luan H., Lee S.M., Brikha A., Ghoreishi-Haack N., Tran L., Stepien I., Aird F., Waters E.A., Yu X., Banks A., Trachiotis G.D., Torkelson J.M., Huang Y., Kozorovitskiy Y., Efimov I.R, & Rogers J.A. (2021). Photocurable bioresorbable adhesives as functional interfaces between flexible bioelectronic devices and soft biological tissues. Nature materials, 20(11), 1559-1570.

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