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Alpha fetoprotein

Manufactured by Abcam
Sourced in United Kingdom

Alpha-fetoprotein is a glycoprotein found in the blood of the fetus and newborn. It is a biomarker used in the diagnosis and monitoring of certain medical conditions, such as liver disease and certain types of cancer.

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2 protocols using alpha fetoprotein

1

Quantifying ALB and AFP Secretion

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To quantify ALB and AFP secretion, organoid culture supernatants were collected after 24 hours and the concentrations of secreted ALB and AFP were measured using Human Albumin (Bethyl Laboratories) and Alpha-fetoprotein (Abcam) ELISA Kits, according to the manufacturers’ instructions. Absorbance was measured using a Spectra Max M3 microplate reader (Molecular Devices).
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2

Immuno-Detection of Cancer Biomarkers

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MWCNTs were purchased from Beijing Nachen S&T Ltd., China. The active human Desmin full-length protein, anti-Desmin antibody, as well as immunoglobulin G (IgG), carcinoembryoic antigen (CEA), and alpha fetoprotein (AFP) were purchased from Abcam PLC (Cambridge, UK). Human peripheral samples were provided by China-Japan Union Hospital of Jilin University, Changchun, Jilin, P.R. China. Other reagents purchased from Sinopharm Chemical Reagent Co, Ltd, China, were analytical grade and directly used without purification. The ultrapure water (18.2 MΩ cm) acquired from a Millipore Milli-Q system was used for preparing solutions.
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