Glucose lactate system solution buffer

For blood glucose measurement, blood samples were collected into heparinized glass capillary tubes and immediately suspended in glucose/lactate system solution buffer (EKF-Diagnostics, Barleben, Germany). Blood glucose was measured using a BIOSEN c-Line glucose meter (EKF-Diagnostics), according to the manufacturer's instructions. For insulin measurement, blood samples were similarly collected in heparinized tubes and plasma was separated and stored at −80°C until analysis. Mouse insulin was measured using the Meso Scale Diagnostics platform.

Blood glucose was measured weekly for the first 4 weeks postsurgery and from there on every third week by collecting blood into heparinized glass capillary tubes and immediate suspension in glucose/lactate system solution buffer (EKF‐diagnostics). Blood glucose was measured using a BIOSEN c‐Line glucose meter (EKF‐diagnostics) according to the manufacturer's instructions. Glycated haemoglobin A1c (HbA1c) levels were measured at termination (16 weeks after surgery) using commercial kits (Roche Diagnostics) on a Cobas^TM C‐501 autoanalyzer (Roche Diagnostics). At study week 15, spot urine samples were collected directly from the penis/vulva to determine the urinary albumin‐to‐creatinine ratio (ACR). Urine creatinine was measured using the CREP2 kit (Roche Diagnostics) on a Cobas^TM C‐501 autoanalyzer. Urinary albumin was measured using a mouse albumin ELISA kit (Bethyl Laboratories, Inc.). Plasma blood urea nitrogen (BUN) levels were measured at termination (16 weeks after surgery) using commercial kits (Roche Diagnostics) on the Cobas^™ C‐501 autoanalyzer.

Blood glucose was measured throughout the study by collecting blood from the tail vein of nonfasted mice into heparinized glass capillary tubes and immediately suspending in glucose/lactate system solution buffer (EKF Diagnostics, Barleben, Germany). Blood glucose was measured using a BIOSEN c-Line glucose meter (EKF Diagnostics) according to the manufacturer's instructions. Spot urine samples were collected 6 and 12 weeks after the injection of viral vector directly from the vulva to determine the urine ACR. Urine creatinine was measured using the CREP2 kit (Roche Diagnostics, Mannheim, Germany) on a Cobas C-501 autoanalyzer. Urine albumin was measured using a Mouse Albumin ELISA Kit (Bethyl Laboratories, Montgomery, TX, USA).