Hair was removed from mouse skin tissue using Veet hair removal cream (Reckitt Benckiser) and the skin washed in Dulbecco's phosphate buffered saline (DPBS) containing 200 U/ml penicillin, 200 U/ml streptomycin, and 5 U/ml amphotericin B1 (all Sigma Aldrich). The skin was then dissected into 2–3 mm2 pieces using a surgical scalpel and 3 or 4 pieces placed per well of a 6‐well dish (Greiner Bio‐One, Kremsmünster, Austria) with the dermis in contact with the dish. 300 µl of DMEM supplemented with 20% FCS, 2 mM L‐glutamine, 200 µg/ml penicillin, 200 µg/ml streptomycin, and 5 µg/ml fungizone (all Sigma Aldrich) was added to the wells. After 24 h, each well was topped up with 1 ml of media, and the media was replenished every 48 h thereafter.
Veet hair removal cream
Manufactured by Reckitt Benckiser
Sourced in Japan, United States, France
Veet hair removal cream is a product designed to remove unwanted body hair. It contains a formula that helps dissolve the hair, making it easy to remove. The cream can be used on various parts of the body, including the legs, arms, and underarms. It is available in different formulas to suit different skin types.
Automatically generated - may contain errors
Lab products found in correlation
Amphotericin b1, by Merck Group (1 mentions)
6 well dish, by Greiner (1 mentions)
Fungizone, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Irdye 800cw protein labeling kit, by LI COR (1 mentions)
Digital camera, by Sony (1 mentions)
Hematoxylin, by StatLab (1 mentions)
Tgf β1 elisa kits, by Cusabio (1 mentions)
Eosin y alcoholic, by StatLab (1 mentions)
Ammonium hydroxide acs reagent, by Merck Group (1 mentions)
Rotary evaporator, by Eyela (1 mentions)
Masson goldner trichrome staining kit, by Merck Group (1 mentions)
31 g needle, by BD (1 mentions)
5 protocols using veet hair removal cream
1
Preparing Mouse Skin Explants
2
Tracking Antigen Migration to Draining Lymph Nodes
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To visualize the migration of antigen to the dLNs, a fluorescent dye-labeled antigen was prepared by conjugating 1 mg OVA protein with 0.1 mg IRDye800CW fluorescent dye using an IRDye800CW protein labeling kit (LI-COR Bioscience). The concentration of the resulting IRDye800CW-labeled OVA (OVA-IR800) was determined using a BCA protein assay. Hair on the left forepaw and the dorsal skin of C57BL/6 mice were removed by applying depilatory creams (VEET Hair Removal Cream; Reckitt Benckiser Japan) for efficient signal transmission. The mice were anesthetized with 3% isoflurane and intradermally administered 25 μg OVA-IR800 alone or mixed with 400 μg alum, 400 μg γ-PGA, or 800 μg PGA/Alum into the forepaw pad. At 1, 3, 6, 24, and 48 h post-injection, in vivo near-infrared (NIR) fluorescent signals from the anesthetized mice were acquired using the in vivo Imaging System (IVIS Lumina II; Xenogen Corp.) with excitation at 780 nm and emission at 831 nm at a 0.02 s exposure time. The fluorescent signals of OVA-IR800 in the axillary lymph node were quantitatively analyzed using image analysis ImageJ software (NIH).
3
Echocardiographic Assessment of Cardiac Function
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Ten months after transplantation, mice were weighed, anesthetized with isoflurane, and intubated as previously described.68 (link) Animals were placed on a heating pad to maintain body temperature at 37°C while being artificially ventilated with a mixture of O2 and N2 (1:2 [v/v]) to which isoflurane (2.5% [v/v]) was added at a rate of 90 strokes/min using a rodent ventilator (SAR-830/P, CWE, Ardmore, PA, USA) at an inspiratory pressure of 18 cm H2O and a positive end expiratory pressure of 4 cm H2O. To dehair the chest, Veet hair removal cream (Reckitt Benckiser, Parsippany, NJ, USA) was used. Transthoracic echocardiograms of the left ventricle were obtained with an Aloka SSD 4000 echo device (Aloka, Tokyo, Japan) using a 13-MHz probe. Images of the short and long axes were obtained in two-dimensional and M-mode settings, used to measure the LV lumen diameter and wall thickness at end diastole and end systole by SigmaScan Pro 5 image analysis software.
4
Wound Healing Promotion Using Herbal Remedies
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Veet hair removal cream was purchased from Reckitt Benckiser (France). White Vaseline was purchased from Korea-ione Co. Ltd. (Gyeonggi-Do, Korea). Silmazin 1 % cream was purchased from Dong-Wha Pharm. Co. Ltd. (Seoul, Korea). Rat interleukin-10 (IL-10) enzyme-linked immunosorbent assay (ELISA) kits and rat transforming growth factor beta 1 (TGF-β1) ELISA kits were purchased from Cusabio Biotech Co. Ltd. (Wuhan, Hubei Province, China). Rat tumor necrosis factor alpha (TNF-α) ELISA kits and rat vascular endothelial growth factor (VEGF) ELISA kits were purchased from Koma Biotech Inc. (Seoul, Korea). The Masson-Goldner trichrome staining kit was purchased from Merck in south Korea (Seoul, Korea), and hematoxylin, eosin Y alcoholic, and Acid Alcohol · HistoTM were purchased from BBC Biochemical Co. (USA). Ammonium hydroxide ACS reagent was purchased from Sigma-Aldrich Co. Inc. (USA), and Harris ethyl alcohol and xylene were purchased from J.T.BakerⓇ (Japan).
In the present study a rotary evaporator (Eyela Co., Japan), ELISA Plate Reader: VersaMax (Molecular Devices Co., USA), digital camera (Sony Corporation, Japan), micro high speed centrifuge (Vision Scientific Co. Ltd., Korea), HM440E microtome (Carl Zeiss, Germany), Olympus DP70 digital microscope camera and Olympus DP controller software (Olympus Imaging America Inc., USA) were used.
In the present study a rotary evaporator (Eyela Co., Japan), ELISA Plate Reader: VersaMax (Molecular Devices Co., USA), digital camera (Sony Corporation, Japan), micro high speed centrifuge (Vision Scientific Co. Ltd., Korea), HM440E microtome (Carl Zeiss, Germany), Olympus DP70 digital microscope camera and Olympus DP controller software (Olympus Imaging America Inc., USA) were used.
5
Mouse Ear Skin Inflammation Induction
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Mice were anaesthetized with an intra-peritoneal injection of ketamine-xylazine (15 mg mL−1 ketamine and 1 mg mL−1 xylazine dissolved in sterile water; 8 μL g−1 bodyweight). Ears were depilated using the Veet hair removal cream (Reckitt Benckiser), and placed on a custom-built platform with regulated heating for the mouse body (36.5 °C) and mouse ear (35 °C). 5 min after the injection, the ear skin was jabbed once using an insulin syringe fitted with a 31G needle (Becton Dickinson), in close proximity to the macroscopically visible post-capillary venules and arterioles.
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