Calbiosorb

All solutions were prepared using dust-free ultrapure deionized water from a Milli-Q® system (Surface tension 72.8 mN.m⁻¹ and resistivity of 18.2 MΩcm @25°C). Magnesium chloride, p-nitrophenylphosphate (pNPP), chloroform, 2-amino-2-methyl-propan-1-ol (AMPOL) were purchased from Sigma Chemical Co. 1,2-dimyristoyl-sn-glycero-3-phosphate (DMPA sodium salt) (99+%) was obtained from AVANTI Polar Lipids. Methanol (MeOH, 99+%) and calcium chloride was purchased from J.T. Baker. Calbiosorb was purchased from Merck-Millipore.

All the solutions were prepared by using dust-free Milli-Q water (γ= 72.8 mN m-1 and resistivity of 18.2 MΩ cm). Sodium dodecylsulfate (SDS), polyoxyethylene-9-lauryl ether (polidocanol, C12(EO)9), potassium bromide, magnesium chloride, sodium chloride, p-nitrophenylphosphate (pNPP), chloroform, 2-amino-2-(hydroxymethyl)propane-1,3-diolhydrochloride (Tris), and 2-amino-2-methyl-propan-1-ol (AMPOL) were purchased from Sigma Chemical Co. [(2R)-2,3-Di(hexadecanoyloxy)propyl] 2-(trimethylazaniumyl)ethyl phosphate (dipalmitoylphosphatidyl-choline, DPPC) (99+%) and (8S,9S,10R,13R,14S,17R)-10,13-dimethyl-17-[(2R)-6-methylheptan-2-yl] 1,2,6,7,8,9,11,12,14,15,16,17-dodecahydrocyclopenta[a]phenanthren-3-one (cholestenone, A-Chol) (99+%) were obtained from AVANTI Polar Lipids. Methanol (MeOH, 99+%) was supplied by J.T. Baker. 3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine (acridine orange, AO) was acquired from Acros Organic. TNAP was obtained from osteoblasts primary cultures as previously reported elsewhere [15 (link)]. Calbiosorb was purchased from Merck-Millipore.