Ethylenediaminetetraacetic acid edta

Manufactured by Scharlab

Sourced in Germany

Ethylenediaminetetraacetic acid (EDTA) is a chelating agent. It is used to bind and remove metal ions from solutions.

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Lab products found in correlation

2 protocols using ethylenediaminetetraacetic acid edta

Oligonucleotide Synthesis and Purification Protocols

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All synthetic oligonucleotides were purchased from Eurofins. PCR primers were desalted, DNAzymes were purified by Eurofins proprietary High Purity Salt Free reverse phase cartridge purification system and short RNA substrates were purified by HPLC. RNase-free ultrapure water was produced using a Milli-Q Advantage A10 Water Purification System equipped with a BioPak® Polisher from Merck Millipore. Phusion High-Fidelity PCR kit and TranscriptAid T7 High Yield Transcription kit were from Life Technologies. 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), sodium chloride, manganese chloride tetrahydrate, formamide, bromophenol blue, 40% 19:1 acrylamide/bis-acrylamide, N,N,N′,N′-tetramethylethylenediamine, ammonium persulfate, urea, agarose, ethidium bromide and phenol:chloroform:isoamyl alcohol 25:24:1 pH 8.0 was purchased from Sigma-Aldrich. Ethylenediaminetetraacetic acid (EDTA) and sodium dodecyl sulfate (SDS) were from Scharlau. Boric acid was from VWR. Klorrent bleach was from Nilfisk. The nucleic acid dyes SYTO 61, PO-PRO 1, DRAQ5, Hoechst 33258, DAPI, Propidium iodide and PicoGreen were all from Life Technologies. GelRed was from Biotium. ethidium bromide was from Sigma.

Eriksson J., Helmfors H, & Langel Ü. (2015). A High-Throughput Kinetic Assay for RNA-Cleaving Deoxyribozymes. PLoS ONE, 10(8), e0135984.

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Comprehensive Analytical Reagent Protocol

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Ammonium acetate, sodium chloride (NaCl), 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), 2-mercaptoethanol, benzamidine, protease inhibitor cocktail, N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), phenylmethanesulfonyl fluoride (PMSF), adenosine 5′-triphosphate (ATP), amino propyl trimethoxy silane (APTS), gluteraldehyde aqueous solution, avidin, N-(+)-Biotinyl-6- aminohexanoic acid (Biotin-X), dipyridamole (DIPY), mesoporphyrin IX (MIX), protoporphyrin IX (PIX), PK-11195 (PK), rotenone (Rot), flunitrazepam (Flu), glipizide, glibenclamide and diclofenac were obtained from Sigma-Aldrich (St. Louis, MO, USA or Munich, Germany), tris(hydroxymethyl)aminomethane (Tris) and glycerol were obtained from Applichem (Darmstadt, Germany), ethylenediaminetetraacetic acid (EDTA) was obtained from Scharlau (Barcelona, Spain). Dialysis tubing was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Open tubular capillaries (100 μm i.d.) were obtained from Polymicro Technologies (Phoenix, AZ, USA). De-ionized water was obtained from a Milli-Q system (Millipore, Molsheim, France). All other chemicals used were of analytical grade.

Habicht K.L., Singh N.S., Indig F.E., Wainer I.W., Moaddel R, & Shimmo R. (2015). The development of mitochondrial membrane affinity chromatography columns for the study of mitochondrial transmembrane proteins. Analytical biochemistry, 484, 154-161.

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