Dp71 ccd camera

The Arabidopisis thaliana was treated by spraying CdTe-QDs (0.8 mmol/L), and the transport and distribution of QDs in the Arabidopisis thaliana organs was observed using laser confocal 2 days later. The samples were observed using a confocal (Olympus FV1000) microscope with 1.4 NA and 60× oil immersion objective. CdTe-QDs were visualized using 488 nm laser excitation and 500 to 550 nm spectral detection. Images were collected at a size of 512 × 512 pixels by using the FV10-ASW 4.2 viewer software. Pollen tubes stained with aniline blue and pollens stained with Alexander dye were observed with fluorescence microscopy (BX53, Olympus, Tokyo, Japan). Images were acquired using an Olympus DP71 CCD camera. Flowers, siliques and seeds were observed with a Zeiss Axio Zoom V16 Materials Stereo Zoom Microscope, and images were acquired using Zeiss Axiocam 506 color camera. Seeds within the silique were observed using an Olympus BX53 fluorescence microscope, and images were acquired using an Olympus DP71 CCD camera. Adobe Photoshop CS5 was used to splice the collected images.

A549 investigated cells were fixed in 3.8% paraformaldehyde solution containing 0.2% Triton X-100 (Sigma-Aldrich, USA) for 5 min at 37°C and subjected to immunostaining. To evaluate the effect of angiotensin-(1–7) and miRNA transfections in A549 cellular morphology, the actin filaments were stained in a 1% bovine serum albumin (BSA) solution containing 100 μg/ ml of phalloidin-tetramethylrhodamine B isothiocyanate (TRITC) (Sigma-Aldrich, USA) for 1 h. Next, the nuclei of the cells were counterstained in 3.33 ng/ ml 4’,6 diamino-2-phenylindole (DAPI, Sigma-Aldrich, USA) solution for 5 min. After extensive washes with saline phosphate buffer (PBS, 2.7 mM KCl, 1.5 mM KH₂PO₄, 137 mM NaCl, and 8 mM Na₂HPO₄, pH 7.4), the coverslips containing cells were subsequently mounted onto slides and subjected to microscopic immunofluorescence analysis. Images were obtained with an Olympus BX51 microscopy equipped with corresponding filter sets and a DP71 CCD camera (Tokyo, Japan). One hundred randomly selected cells were analyzed in each investigated group. For phase-contrast analyses required in the in vitro scratching assays (wound healing) and in the cellular invasion chamber assays, the same microscopy was used and images were monitored and quantified using the Image J software (http://rsb.info.nih.gov/ij/).

Whole-mount images were acquired with a Leica MZFL III binocular microscope coupled to an Olympus DP 71 CCD camera and Olympus cellSense software. Bright-field images were obtained with an Olympus BX51 Microscope and Olympus cellSense software. Confocal images were acquired with a Nikon A1R confocal microscope. For in vivo confocal imaging, zebrafish embryos and larvae were mounted and anaesthetized in 1% low-melting agarose (Sigma-Aldrich, A9414) containing 0.2% (w/v) tricaine on glass-bottom dishes. Z-plane images were obtained with a spinning disc confocal microscope (Zeiss, CSU-X1 Yokogawa) fitted with a 40× [1.1 numerical aperture (NA)] water immersion objective. The optical section thickness was 1 μm. Three nonconsecutive single-plane images per larvae were taken using a Zeiss LSM780 confocal microscope and a 40× (1.1 NA) water immersion objective. Confocal data were processed with ZEN 2012 software (black edition), and images were analyzed with ImageJ.