Primary antibodies used for immunostaining and Western blotting: anti-phospho-Histone S139 (γ-H2AX) and anti-β-tubulin (Cell Signaling), anti-BRCA1 (Calbiochem), anti-RBL2 (BD Transduction Laboratories). Secondary antibodies for immunostaining: goat anti-rabbit CFL488 (Santa Cruz) and goat anti-rabbit Alexa Fluor 488 (Life Technologies). NO donors SNAP and DETA NONOate (DETA) were purchased from Cayman Chemical. FlexiTube siRNA mixtures (Qiagen) for siRNA transfection included Hs_BRCA1_13 (SI02654575), Hs_RBL2_6 (SI02664473), and AllStars non-coding negative control (SI03650318). Transfections with 50 nMol/L siRNAs were conducted according to the manufacturer's recommendations.
Anti rbl2
Manufactured by BD
Anti-RBL2 is a laboratory reagent used to detect and quantify the expression of the RBL2 (Retinoblastoma-Like 2) protein. It is a specific antibody that binds to the RBL2 protein, allowing researchers to analyze its presence and levels in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase secondary, by Santa Cruz Biotechnology (1 mentions)
Anti fas, by Merck Group (1 mentions)
Anti tp73, by Santa Cruz Biotechnology (1 mentions)
Anti tp53, by Santa Cruz Biotechnology (1 mentions)
Imagequant las 4000, by GE Healthcare (1 mentions)
Anti actin, by Santa Cruz Biotechnology (1 mentions)
Protease inhibitor, by Roche (1 mentions)
2 protocols using anti rbl2
1
Immunostaining and Western Blotting
2
Protein Expression Analysis of HosDXR150 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Treated and untreated HosDXR150 cells were lysed in 50 mM Tris/HCl, 5 mM EDTA, 250 mM NaCl, 50 mM NaF, 0.1% Triton-X, 0.1 mM Na3VO4 plus protease inhibitors (Roche Molecular Biochemicals, Germany). Equal amounts of proteins were resolved on a 7% or 10% SDS-PAGE, transferred onto nitrocellulose membranes, and then successively blocked with 5% non-fat dry milk. Anti-RBL2 was from BD Transduction Laboratories. Anti-FAS, anti-CASP10 and anti-IL12A were from Sigma-Aldrich. Anti-TP73, anti-TP53, anti-actin and the horseradish peroxidase secondary antibodies were from Santa Cruz Biotechnology. Primary antibodies were used at a dilution of 1∶200. Secondary antibodies were used at a dilution of 1∶5000. Signals were acquired using the ImageQuant LAS 4000 (GE Healthcare).
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