Plano convex

Manufactured by Thorlabs

Plano-convex lenses are optical components with a flat (planar) surface on one side and a curved (convex) surface on the other. They are used to refract light and are commonly employed in various optical systems to perform tasks such as collimation, focusing, and beam expansion.

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Lab products found in correlation

Uplsapo, by Olympus (1 mentions) Sepia 2 multichannel processor, by PicoQuant (1 mentions) La 1951 a, by Thorlabs (1 mentions) La1509 a, by Thorlabs (1 mentions) Ff495 di03, by IDEX Corporation (1 mentions) Maya2000 pro, by OceanOptics (1 mentions) Eclipse ti2 e inverted microscope, by Nikon (1 mentions) Perfect focusing system, by Nikon (1 mentions)

3 protocols using plano convex

3D Holographic Tracking of Sperm Cells

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Freely swimming sperm were tracked using an inverted microscope (IX71; Objective × 20, 0.75 numerical aperture, UPLSAPO; Olympus). Coherent illumination was achieved with a laser light source (LDH-D-C-510, PicoQuant GmbH) and the corresponding controller (Sepia II Multichannel Processor, PicoQuant GmbH). Laser light was coupled into a multimode fibre. A custom-made adapter was used to position the fibre parallel to the optical axis of the objective. The illumination intensity was adjusted to use the dynamical range of the camera (12-bit; PCO Dimax HD). Movies were collected at 600 frames per second; each frame represents a holographic image containing the complete 3D information of the sperm cell.
Caged compounds were photolyzed using a 365-nm LED (M365L2-C; Thorlabs). The ultraviolet light was coupled into a liquid guide (77566; Newport) followed by two Plano-convex lenses (LA 1951-A f=25.4 mm; LA 1509-A f=100 mm; Thorlabs) and coupled to the imaging optical path with a dichroic filter (ff 495-Di03; Semrock). The irradiation power (0.8 mW) was measured with a power meter (detector PowerMax and head model PS19Q; Coherent). The light spectrum was recorded with a spectrometer (51024 DW; Ocean Optics). Photolysis and data acquisition were synchronized using a wave generator (33500B; Agilent).

Jikeli J.F., Alvarez L., Friedrich B.M., Wilson L.G., Pascal R., Colin R., Pichlo M., Rennhack A., Brenker C, & Kaupp U.B. (2015). Sperm navigation along helical paths in 3D chemoattractant landscapes. Nature Communications, 6, 7985.

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Total Internal Reflection Spectroscopy

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In this experiment, the light source was directed toward the interface from underneath (through the organic phase) with the aid of focusing lenses, diaphragm, and mirrors; see schematic in Fig. 6. An angle of incidence (AOI) of ca. 75° was used to ensure TIR conditions (see optical image in Fig. 6), as θ₁ was calculated as 70.05° using the Snell’s law (ηTFT sinθ₁ = ηH₂O sinθ₂; where ηTFT = 1.414, ηH₂O = 1.330, and θ₂ is assumed to be 90°).
The light source (Xe lamp HPX-2000, Ocean Optics) was guided by an optical fiber with a 200-μm core (Newport) and focused on the water-TFT interface through plano-convex (Thorlabs) and achromatic lenses (Newport); see Fig. 6. All lenses were placed at their confocal lengths. The longer wavelengths (λ > 700 nm) were cut by a Hot Mirror (Thorlabs) to avoid heating of the interfacial region. The reflected light was focused onto an optical fiber with a 1500 mm core (Thorlabs). The absorption spectra were recorded by a Maya 2000Pro (Ocean Optics).

Gamero-Quijano A., Bhattacharya S., Cazade P.A., Molina-Osorio A.F., Beecher C., Djeghader A., Soulimane T., Dossot M., Thompson D., Herzog G, & Scanlon M.D. (2021). Modulating the pro-apoptotic activity of cytochrome c at a biomimetic electrified interface. Science Advances, 7(45), eabg4119.

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Super-Resolution Imaging of Single Molecules

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Samples were imaged using an ECLIPSE Ti2-E inverted microscope (Nikon). Lasers were housed in a C-FLEX laser combiner (HÜBNER Photonics), containing 405 nm (06 series, HÜBNER Photonics), 488 nm (06 series, HÜBNER Photonics), 561 nm (04 series HÜBNER Photonics), and 638 nm (06 series, HÜBNER Photonics) lasers. These were all aligned inside the combiner and were coupled to the E-TIRF arm (Nikon). A filter cube containing a dichroic mirror, in addition to bandpass and longpass filters for all four laser lines (C-NSTORM QUAD 405/488/561/647 FILT; Nikon) was installed inside a motorized filter turret below a CFI Apochromat TIRF 100XC NA 1.49 Oil objective (Nikon). Samples were placed on a motorized stage, and a Perfect Focusing System (Nikon) was used to minimize drift in the z direction. Images were recorded by an sCMOS camera (Prime95B, Photometrics) with a pixel size of 11 × 11 μm, at 20 Hz. Super-resolution (SMLM) images were reconstructed from 2,000 frames, and diffraction-limited images of aggregates on coverslips were averaged from 100 frames. In order to record axial information of single molecules, we placed a cylindrical lens (f = 1,000.0 mm, Plano-Convex; Thorlabs) in the Optosplit II and followed the astigmatism method of 3D SMLM (89 (link)).

Morten M.J., Sirvio L., Rupawala H., Mee Hayes E., Franco A., Radulescu C., Ying L., Barnes S.J., Muga A, & Ye Y. (2022). Quantitative super-resolution imaging of pathological aggregates reveals distinct toxicity profiles in different synucleinopathies. Proceedings of the National Academy of Sciences of the United States of America, 119(41), e2205591119.

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