RNA was isolated from neurons using Trizol reagent following the manufacturer’s protocols. cDNA was generated using superscript IV following the manufacturers’ protocols with 2 μg of total RNA and using random hexamer primers. PCR was performed using GoTaq PCR mastermix (Promega). Quantitative PCR (qPCR) was performed using Power SYBR green and an MX3000P thermocycler (Agilent). Primers are shown in Table 1 .
Power sybr green
Manufactured by Agilent Technologies
Power SYBR green is a fluorescent dye used in real-time PCR (polymerase chain reaction) experiments to detect and quantify DNA. It binds to double-stranded DNA, and its fluorescence increases upon binding, allowing for the monitoring of DNA amplification during the PCR process.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using power sybr green
1
RNA Extraction and qPCR Analysis
2
Neuron RNA Isolation and Quantitative PCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA was isolated from neurons using Trizol reagent following the manufacturer’s protocols. cDNA was generated using superscript IV following the manufacturers’ protocols with 2 µg of total RNA and using random hexamer primers. PCR was performed using GoTaq PCR mastermix (Promega). Quantitative PCR (qPCR) was performed using Power SYBR green and an MX3000P thermocycler (Agilent). Primers are shown in Table 1 .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!