Dihydrotestosterone

Manufactured by Merck Group

Sourced in United States, United Kingdom, Germany, China

Dihydrotestosterone is a synthetic organic compound that is an androgen and anabolic steroid. It is primarily used in research and laboratory settings for scientific analysis and experimentation purposes.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Dihydrotestosterone (DHT) (60 citations) 5α-dihydrotestosterone (DHT) (30 citations) 5α-dihydrotestosterone (8 citations) 5α-dihydrotestosterone (5α-DHT) (4 citations) 5α-Dihydrotestosterone (DHT) solution (2 citations) Mol/L dihydrotestosterone (1 citations)

58 protocols using dihydrotestosterone

Platelet Activation Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

LC–MS grade, acetonitrile (MeCN), methanol (MeOH), tetrahydrofuran (THF), dimethyl sulfoxide, MS grade formic acid (HCOOH) and all the analytical standards of testosterone, dihydrotestosterone, α-estradiol, β-estradiol, methyltestosterone, as well as the hormone preparations used for in vitro measurements of platelet activation/reactivity were obtained from Sigma (St. Louis, MO, USA) and had a minimum purity specification of 99%. Nitric acid (HNO₃) was purchased from POCH (Gliwice, Poland). Dichloromethane (DCHM, HPLC grade) was provided by VWR International (Radnor, PA, USA).
PBS was from Avantor Performance Materials Poland S.A. (Gliwice, Poland). Arachidonate, equine tendon collagen and ADP were from Chrono-Log Corp. (Havertown, PA, USA). Fluorolabelled monoclonal antibodies (moAbs): anti-CD61/PerCP, antiCD62/PE, PAC-1/FITC, isotype controls IgG/PE and IgM/FITC, as well as CellFix (1% formaldehyde in PBS) were from Becton Dickinson (San Diego, CA, USA). Ultrapure water was obtained from Milli-Q purification system (Millipore, Bedford, MA, USA). Nitrogen (NM32LA Nitrogen Generator, Peak Scientific Instruments, Billerica, MA, USA) was used as a drying gas.

Karolczak K., Konieczna L., Kostka T., Witas P.J., Soltysik B., Baczek T, & Watala C. (2018). Testosterone and dihydrotestosterone reduce platelet activation and reactivity in older men and women. Aging (Albany NY), 10(5), 902-929.

+ Open protocol

+ Expand

Steroid Biomarker Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Calibrator stock solutions were prepared separately for each steroid by dissolving reference steroids in ethyl acetate. The stock solutions were then pooled to a calibrator standard solution and diluted in 50% methanol/ water. Eight calibrator working solutions were made within the calibration range presented in Supplementary Table 1 (4 (link)). Estradiol, estrone, dihydrotestosterone, progesterone, androstenedione, DHEA, and 17α-hydroxyprogesterone were purchased from Sigma-Aldrich (St. Louis, MO, USA). Testosterone was purchased from Fluka (Buchs, Germany). Calibration was performed by determining the peak area ratio between the target analyte and the isotope-labeled internal standard. Before each run, calibrator samples were freshly prepared by spiking 25 µL calibrator working solution to 225 µL of 0.5% BSA in PBS buffer. Internal standard stock solutions were made separately using ¹³C₃-labeled versions of each steroid, except for DHEA that was labeled with d6. An internal standard working solution with labeled steroid concentrations in the middle of the calibration range was prepared in 50% methanol/water and aliquoted. All calibrator and internal standard stock and working solutions were stored at −80 °C until use.

Ohlsson C., Langenskiöld M., Smidfelt K., Poutanen M., Ryberg H., Norlén A.K., Nordanstig J., Bergström G, & Tivesten Å. (2021). Low Progesterone and Low Estradiol Levels Associate With Abdominal Aortic Aneurysms in Men. The Journal of Clinical Endocrinology and Metabolism, 107(4), e1413-e1425.

+ Open protocol

+ Expand

Prostate Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols

LNCaP, MDA-PCa-2b, and PC3 cell lines were acquired from ATCC (Manassas, VA, USA). PC3 and LNCaP cells lines were cultured in 10% fetal bovine serum (FBS), RPMI 1640, and 1% antibiotic-antimycotic. The MDA-PCa-2b cell line was cultured in BRFF-HPC1 media purchased from AthenaES (Baltimore, MD, USA) supplemented with 20% FBS. Dihydrotestosterone was acquired from Sigma-Aldrich (St. Louis, MO, USA). Androgen depleted media consisted of 10% charcoal stripped FBS with phenol red free RPMI 1640. Docetaxel was acquired from Sigma-Aldrich. Temsirolimus and SB202190 were purchased from Selleckchem (Houston, TX, USA). All other inhibitors were purchased from EMD Millipore (Billerica, MA, USA). Unless otherwise stated all other cell culture reagents were acquired from Invitrogen (Grand Island, NY, USA).

Lescarbeau R, & Kaplan D.L. (2014). Correlating phosphoproteomic signaling to castration resistant prostate cancer survival through regression analysis. Molecular bioSystems, 10(3), 605-612.

+ Open protocol

+ Expand

Biosynthesis and Evaluation of Depsidones

Check if the same lab product or an alternative is used in the 5 most similar protocols

The depsidones Unguinol and Aspergillusidone D were provided by the Chulabhorn Research Institute and have been biosynthesized according to Sureram et al. [12 ]. The other compounds tested, i.e. 17β-estradiol (purity >98%), testosterone C-III (purity >98%), 5α-androstan-17β-ol-one C-III or dihydrotestosterone (purity 97.5%), colchicine (purity ±95%) and doxorubicin (purity >98%) were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). These compounds were dissolved and diluted in dimethyl sulphoxide (DMSO) Hybri-max from Sigma-Aldrich (St. Louis, MO, USA) and added to the exposure medium at <0.5% (v/v).

Zwartsen A., Chottanapund S., Kittakoop P., Navasumrit P., Ruchirawat M., Van Duursen M.B, & Van den Berg M. (2019). Evaluation of anti-tumour properties of two depsidones – Unguinol and Aspergillusidone D – in triple-negative MDA-MB-231 breast tumour cells. Toxicology Reports, 6, 1216-1222.

+ Open protocol

+ Expand

Metastatic Pancreatic Cancer Organoid Establishment

Check if the same lab product or an alternative is used in the 5 most similar protocols

For identifying the HM-SE genes, 2 patients with PT and 2 patients with HM were enrolled for H3K27ac ChIP-Seq analysis (Table S1). The samples were obtained from Ruijin Hospital, Shanghai Jiaotong University School of Medicine. The study protocol was approved by the Research Ethics Committee of Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University. All enrolled participants consented to attend this cohort study and signed written informed consent. To create the metastatic patient-derived organoids (MDO), the fresh ccPDAC tissues were enzymatically digested and then treated for 1 hour at 37°C with 200 U/ml of deoxyribonuclease I (Roche) and collagenase type IV (SigmaAldrich) (Table S1). The cells plated in Basement Membrane (OuMel, #WM-MG-01) were filtered using a 70-m nylon mesh and and cultured by DMEM/F12 media containing 2% B27, N-acetyl-l-cysteine (SigmaAldrich, 1.25 mM), EGF (Gibco, 50 ng/mL), A83-01 (SigmaAldrich, 200 nM), Noggin (SigmaAldrich, 100 ng/mL), R-spondin 1 (SigmaAldrich, 500 ng/mL), Y-27632 (MedChemExpress, 10 mM), and dihydrotestosterone (SigmaAldrich, 1 nM).

Chen D., Cao Y., Tang H., Zang L., Yao N., Zhu Y., Jiang Y., Zhai S., Liu Y., Shi M., Zhao S., Wang W., Wen C., Peng C., Chen H., Deng X., Jiang L, & Shen B. (2023). Comprehensive machine learning-generated classifier identifies pro-metastatic characteristics and predicts individual treatment in pancreatic cancer: A multicenter cohort study based on super-enhancer profiling. Theranostics, 13(10), 3290-3309.

+ Open protocol

+ Expand

Prostate Cancer Compound Evaluation

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Apalutamide, darolutamide, dexamethasone, enzalutamide, mifepristone, OTX015, and JQ1 were purchased from Selleckchem. R1881, dihydrotestosterone (DHT), and beta-estradiol were purchased from Sigma-Aldrich. BET protein degrader ZBC260 and AR protein degrader ARD-61 were described previously (28 (link), 29 (link)).

Qiao Y., Wang X.M., Mannan R., Pitchiaya S., Zhang Y., Wotring J.W., Xiao L., Robinson D.R., Wu Y.M., Tien J.C., Cao X., Simko S.A., Apel I.J., Bawa P., Kregel S., Narayanan S.P., Raskind G., Ellison S.J., Parolia A., Zelenka-Wang S., McMurry L., Su F., Wang R., Cheng Y., Delekta A.D., Mei Z., Pretto C.D., Wang S., Mehra R., Sexton J.Z, & Chinnaiyan A.M. (2020). Targeting transcriptional regulation of SARS-CoV-2 entry factors ACE2 and TMPRSS2. Proceedings of the National Academy of Sciences of the United States of America, 118(1), e2021450118.

+ Open protocol

+ Expand

Stable Cell Lines for SLX4IP Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

All cell lines were maintained at 37°C in 5% CO₂ following acquisition from ATCC. LAPC4 cells were grown in IMDM (Sigma) supplemented with 10% (v/v) FBS (Sigma) and 1 nM dihydrotestosterone (Sigma). C4–2B, CWR-R1, 22Rv1, DU145, and PC-3 cells were grown in RPMI1640 (Gibco) supplemented with 10% FBS and 1% of Penicillin 10 000 units/mL, Streptomycin 10 000 μg/mL, and 25 μg/mL Amphotericin B mixture (Antibiotic-Antimycotic, Gibco). HEK293T, HCT116, and U2OS cells were grown in DMEM (Gibco) supplemented with 10% (v/v) FBS (Sigma) and 1% Antibiotic-Antimycotic (Gibco). All cell lines were tested for Mycoplasma using the MycoAlert™ PLUS Detection Kit (Lonza) and STR validated (CWRU Genomics Core) prior to experimental testing and following each genetic modification.
Generation and selection of stable cell lines overexpressing SLX4IP and SLX4IP truncation constructs utilized an identical strategy as described previously.^{35 (link)} To generate the 3XFLAG-tagged SLX4IP truncation constructs depicted in Figure 1a, full-length 3XFLAG-tagged SLX4IP was sub-cloned by TOP Gene Technologies, Inc. Generation and selection of stable cell lines with SLX4IP knockdown utilized an identical strategy as described previously.^{35 (link)}

Mangosh T.L., Grabowska M.M, & Taylor D.J. (2021). SLX4IP N-terminus dictates telomeric localization in ALT-like castration-resistant prostate cancer cell lines. The Prostate, 81(15), 1235-1251.

+ Open protocol

+ Expand

Testosterone Propionate Procurement and Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

Testosterone propionate (TP) was procured from the Tokyo Chemical Industry Co. (Tokyo, Japan). Protease inhibitor cocktail, finasteride (Fi) (≥97% pure), and dihydrotestosterone (≥99% pure) were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Roswell Park Memorial Institute (RPMI) medium, fetal bovine serum (FBS), and penicillin/streptomycin were purchased from Gibco (Big Cabin, OK, USA).

Choi Y.J., Lee J.I., Fan M., Tang Y., Yoon E.J., Ryu Y.B, & Kim E.K. (2020). Metabolomic Analysis of Morus Cultivar Root Extracts and Their Ameliorative Effect on Testosterone-Induced Prostate Enlargement in Sprague-Dawley Rats. International Journal of Molecular Sciences, 21(4), 1435.

+ Open protocol

+ Expand

Prostate Organoid Culture Protocol

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The organoid culture was performed as described previously (Karthaus et al., 2014 (link)). Briefly, dissociated prostate cells from 8-12 week-old C57B1/6 mice were cultured in DMEM/F12 supplemented with B27 (Life technologies, Grand Island, NY), 10 mM HEPES, Glutamax (Life technologies, Grand Island, NY), Penicillin/Streptomycin, and the following growth factors: EGF 50 ng/ml (Peprotech, Rocky Hill, NJ), 500 ng/ml recombinant R-spondin1 (Peprotech, Rocky Hill, NJ), 100 ng/ml recombinant Noggin (Peprotech, Rocky Hill, NJ), 200 μM TGF-β/Alk inhibitor A83-01 (Tocris, Ellisville, MO), and 10 μM Y-27632 (Tocris, Ellisville, MO). Dihydrotestosterone (Sigma, St. Louis, MO) was added at 1 nM final concentration. Cells were resuspended in growth factor reduced matrigel (Corning, Corning, NY) and plated in 96-well plates.

Wei X., Zhang L., Zhou Z., Kwon O.J., Zhang Y., Nguyen H., Dumpit R., True L., Nelson P., Dong B., Xue W., Birchmeier W., Taketo M., Xu F., Creighton C.J., Ittmann M.M, & Xin L. (2019). SPATIALLY-RESTRICTED STROMAL WNT SIGNALING RESTRAINS PROSTATE EPITHELIAL PROGENITOR PROLIFERATION THROUGH DIRECT AND INDIRECT MECHANISMS. Cell stem cell, 24(5), 753-768.e6.

+ Open protocol

+ Expand

Enzalutamide, DHT, and JQ-1 Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Enzalutamide was obtained from MedchemExpress (HY-70002). Dihydrotestosterone (DHT) was obtained from Sigma (A8380). For in vitro experiments, JQ-1 was obtained from BPS Bioscience (27402). For in vivo studies, JQ-1 was obtained from the laboratory of Dr. James Bradner, Dana Farber Cancer Institute Dept. of Medical Oncology/Harvard Medical School Dept. of Medicine. For in vitro studies, DMSO stocks of Enzalutamide and JQ-1 and ethanol stocks of DHT were diluted to desired working concentrations in cell culture media (RPMI with 10% charcoal-stripped fetal bovine serum unless otherwise noted) with a final vehicle concentration of 0.1%. Vehicle-only controls were used in all cases, and vehicle concentrations were normalized across all drug co-treatments. See below for information on administration of drugs for in vivo studies.

Coleman D.J., Van Hook K., King C.J., Schwartzman J., Lisac R., Urrutia J., Sehrawat A., Woodward J., Wang N.J., Gulati R., Thomas G.V., Beer T.M., Gleave M., Korkola J.E., Gao L., Heiser L.M, & Alumkal J.J. (2016). Cellular androgen content influences enzalutamide agonism of F877L mutant androgen receptor. Oncotarget, 7(26), 40690-40703.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!