Fetal BSA (GIBCO), heat-passivated FBS (Invitrogen), heat-inactivated FCS (Invitrogen), and 1-oleoyl LPA (Tocris Bioscience) were used at the indicated concentrations (1, 5, 10, and 50 nM). Pharmacological inhibitors were used at the following concentrations: 10 μM active (−) or inactive (+) Bb (Tocris Bioscience), 0.3 μM Latrunculin-B (Sigma-Aldrich), 100 nM Jasp (Invitrogen), and 2 mg/mL Concavalin A (Sigma-Aldrich).
Fetal bsa
Manufactured by Thermo Fisher Scientific
Fetal BSA is a laboratory reagent used in various biomedical applications. It is a purified form of bovine serum albumin derived from fetal bovine sources. Fetal BSA provides a consistent and reliable source of protein for use in cell culture media, assays, and other laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Latrunculin b, by Merck Group (2 mentions)
Concavalin a, by Merck Group (2 mentions)
Blebbistatin, by Bio-Techne (2 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (2 mentions)
Latrunculin a, by Merck Group (1 mentions)
Ml141, by Merck Group (1 mentions)
1 oleoyl lysophosphatidic acid lpa, by Bio-Techne (1 mentions)
Jasplakinolide, by Bio-Techne (1 mentions)
Pdgf aa, by Thermo Fisher Scientific (1 mentions)
Human epidermal growth factor (hegf), by Merck Group (1 mentions)
Y 27632, by Bio-Techne (1 mentions)
Jasplakinolide, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
3 protocols using fetal bsa
1
Cell Culture Reagents and Inhibitors
2
Cell Polarity Modulation Assay
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Fetal BSA (GIBCO) and 1-Oleoyl lysophosphatidic acid (LPA, Tocris Bioscience) were used at the indicated concentrations. Fibroblast growth factor mFGF-8b and rhFGF-3 (R&D Bioscience), platelet-derived growth factor PDGF-AA (PeproTech), and epidermal growth factor hEGF (Sigma) were used at a final concentration of 100 ng/μl. Pharmacological inhibitors were used at the following concentrations: 10 μM active (−) or inactive Blebbistatin (+) (Tocris Bioscience), 100 nM Latrunculin-A (Sigma), 100 μM Y-27632 (Tocris Bioscience), 500 nM Jasplakinolide (Tocris Bioscience), 20 μM ML-141 (Sigma), and 30 μM L-294002 (Sigma). The percentage of polarized cells was measured after 15 min (Blebbistatin, LatrunculinA, and Jasplakinolide) and 30–60 min (Y-27632, ML-141, Y-294002) of exposure.
3
Fetal Cell Culture Optimization
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Fetal BSA (GIBCO), heat-passivated FBS (Fetal Bovine Serum, Invitrogen), heat-inactivated FCS (Fetal Calf Serum, Invitrogen), and 1-Oleoyl lysophosphatidic acid (LPA, Tocris Bioscience) were used at the indicated concentrations (1 nM, 5 nM, 10 nM and 50 nM, respectively). Pharmacological inhibitors were used at the following concentrations: 10 μM active (-) or inactive Blebbistatin (+) (Tocris Bioscience), 0.3 μM Latrunculin-B (Sigma-Aldrich), 100 nM Jasplakinolide (Invitrogen), and 2 mg/mL Concavalin A (Sigma-Aldrich).
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