The 1H (600 MHz/800 MHz) and 13C (600 MHz/800 MHz) NMR spectra were recorded using a Bruker AVANCE III 600 MHz Spectrometer (1H NMR spectra parameter configuration: NS = 4, DS = 2, DW = 41.6 usec. 13C NMR spectra parameter configuration: NS = 4/1024, DS = 2/4, DW = 41.6/13.8 usec.) and Bruker AV 800MHz Spectrometer (1H NMR spectra parameter configuration: NS = 4, DS = 0, DW = 31.2 usec. 2D NMR spectra parameter configuration: NS = 8/16, DS = 16/16, DW = 56.8/59.4 usec) (Bruker, Billerica, MA, USA) in a deuterated solvent. HR-ESI-MS and ESI-MS analyses were performed using a Shimadzu Corporation UPLC-IT-TOF spectrometer (Shimadzu, Kyoto, Japan). Preparative high-performance liquid chromatography was performed on a DAC-HB50 separation module combined with a UV detector at 535 and 280 nm (Hanbon, Huai’an, China). A C18-ODS-A (300 mm × 50 mm, 5 μm, YMC, Kyoto, Japan) and XAqua C18 semi-preparative column (4.6 × 250 mm, 5 μm, Acchrom, Wenling, China) were used for separation. UV spectra were acquired using a T6 New Century spectrophotometer (Persee, Beijing, China). Microscopic observation and photographs were taken using a SZX7 dissecting microscope (Olympus, Tokyo, Japan) and a CCD camera (VertA1, Shanghai, China). All chemicals and solvents were of chromatographic grade.
Szx7 dissecting microscope
Manufactured by Olympus
Sourced in Japan
The SZX7 dissecting microscope is a high-performance optical instrument designed for detailed observation and examination. It features advanced optics and a compact, ergonomic design to provide clear, high-resolution views of specimens. The SZX7 is a versatile tool suitable for a variety of applications that require detailed visual inspection.
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Lab products found in correlation
Av800 mhz spectrometer, by Bruker (1 mentions)
Uplc it tof spectrometer, by Shimadzu (1 mentions)
Avance 3 600 mhz spectrometer, by Bruker (1 mentions)
C18 ods a, by YMC (1 mentions)
Ccd camera, by Olympus (1 mentions)
Dp72 digital camera, by Olympus (1 mentions)
Cell f software, by Olympus (1 mentions)
Vhx 2000 digital microscope, by Keyence (1 mentions)
5 protocols using szx7 dissecting microscope
1
Multi-Spectroscopic Characterization of Compounds
2
Crawling Speed Measurement in Animals
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The speed of crawling animals was measured on a fresh NGM plate. Day 1-stage adult animals were moved onto an NGM plate without food and allowed to acclimate for 10 seconds. Digital videos of animal movement were acquired using an Olympus SZX7 dissecting microscope (Center Valley, PA) equipped with a 3.2 Megapixel OLYMPUS Q-Color3 digital camera (Melville, NY) and Q-Capture Pro 7 imaging software. The videos were recorded at 1 x 1 binning for 500 frames at 20 frames per second. The videos were analyzed using the open-source wrMTrck plugin for ImageJ software available publicly on the internet (http://www.phage.dk/plugins/wrmtrck.html ). At least 30 animals per genotype were analyzed.
3
Collecting and Preserving Gudgeon Specimens
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In October 2012, November 2013 and May 2015, a total of 52 S. gudgeri were collected from Jinghong Basin, a main tributary of the international Lancang-Mekong River in southwest China. The taxonomic status of fish specimens was determined according to the checklist of fishes and fauna of Yunnan Province [6 (link)]. Fish were euthanized by severing the spinal cord posterior to the skull with a single cut [6 (link), 7 ]. Gills were removed and examined under an Olympus SZX7 dissecting microscope (Olympus, Japan). Specimens were washed in double-distilled water before being preserved in either 70% or 100% ethanol for morphological and molecular research, respectively.
4
Constructing Transcriptional Fusion Reporters
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A plasmid harboring a fusion between the M. xanthus mxcG gene and lacZ was constructed using vector pKY481 [53] and the oligonucleotides listed in Table S2 as primers. The BamHI site in the primer was introduced at the start codon of the M. xanthus gene and in frame with the BamHI site existing in the lacZ gene of plasmid pKY481. This plasmid was introduced into M. xanthus by electroporation, and the strains were selected as previously described [49] .
To obtain a transcriptional fusion of the S. meliloti promoter for the rhtXrhbABCDEF operon to lacZ, a DNA fragment upstream of rhtX was PCR amplified using Sm_WT genomic DNA and the primers listed in Table S2. The resulting amplicon was cloned into pMP220 to give the plasmid pMPrhBIO, which was used to generate strains harboring the rhb-lacZ fusion (Table S1).
For β-galactosidase activity analyses, M. xanthus and S. meliloti were treated as shown in Figure S2A. Blue color development, resulting from β-galactosidase activity in representative samples, was recorded using an Olympus (Tokyo, Japan) SZX7 dissecting microscope equipped with a DP72 digital camera and analyzed using the Olympus Cell^F software. β-galactosidase specific activity of predator and prey in pure cultures was quantified as previously reported [49] .
To obtain a transcriptional fusion of the S. meliloti promoter for the rhtXrhbABCDEF operon to lacZ, a DNA fragment upstream of rhtX was PCR amplified using Sm_WT genomic DNA and the primers listed in Table S2. The resulting amplicon was cloned into pMP220 to give the plasmid pMPrhBIO, which was used to generate strains harboring the rhb-lacZ fusion (Table S1).
For β-galactosidase activity analyses, M. xanthus and S. meliloti were treated as shown in Figure S2A. Blue color development, resulting from β-galactosidase activity in representative samples, was recorded using an Olympus (Tokyo, Japan) SZX7 dissecting microscope equipped with a DP72 digital camera and analyzed using the Olympus Cell^F software. β-galactosidase specific activity of predator and prey in pure cultures was quantified as previously reported [49] .
5
Collection and Morphological Analysis of a New Insect Species
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The type specimens were collected by sweep net in Aug 2008 in the Baiyun Mountain scenic area in Henan Province. Morphological terminology used in this work follows Dietrich (2005) and Song & Li (2013) . Habitus photos were taken using a KEYENCE VHX-2000 digital microscope. The body measurements are from the apex of vertex to the tip of forewing. An Olympus SZX7 dissecting microscope was used for viewing and an Olympus CX41 stereoscopic microscope for drawing. The type-series of the new species were deposited in the Institute of Entomology, Guizhou University, Guiyang, China (GUGC).
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