2 meoe2

Manufactured by Merck Group

Sourced in United States

2-MeOE2 is a laboratory chemical compound. It is a methylated derivative of 2-ethoxystilbene. The core function of 2-MeOE2 is as a research tool for scientific investigations. No further details on its intended use can be provided in an unbiased and factual manner.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) H1299, by Merck Group (1 mentions) H1299, by BNCC (1 mentions) Anti mir 34a 5p, by GenePharma (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Anti mir nc, by GenePharma (1 mentions) Mda mb 435, by National Collection of Authenticated Cell Cultures (1 mentions) Luciferase assay kit, by Promega (1 mentions) Glomax 96 microplate luminometer, by Promega (1 mentions)

3 protocols using 2 meoe2

Lung Cancer Cells Transfection Protocol

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Lung cancer cells (H1299 and A549) and human bronchial epithelioid cells (HBE) were obtained from the BeNa Culture Collection (Beijing, China). These cells were propagated in Dulbecco's modified Eagle medium (DMEM: Invitrogen) mixed with 1% penicillin/streptomycin (Invitrogen) and 10% fetal bovine serum (Gibco) at an atmosphere of 37°C with 5% CO₂. H1299 and A549 cells were treated with 2‐MeOE2 (Sigma‐Aldrich).
Cells were transfected with lipofectamine 3000 (Invitrogen). All plasmids and oligonucleotides including the circ_0010235 overexpression vector (circ_0010235) and its control pCD5‐ciR, the small interfering RNA (siRNAs) targeting circ_0001955 (si‐circ_0001955) and si‐NC, miR‐34a‐5p mimics, miR‐NC, miR‐34a‐5p inhibitor (anti‐miR‐34a‐5p), anti‐miR‐NC, NFAT5‐overexpressing plasmid (NFAT5) and matching negative control (pcDNA) were synthesized from GenePharma.

Zhang Y., Mi Y, & He C. (2023). 2‐methoxyestradiol restrains non‐small cell lung cancer tumorigenesis through regulating circ_0010235/miR‐34a‐5p/NFAT5 axis. Thoracic Cancer, 14(22), 2105-2115.

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Culturing Human Melanoma Cells

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Human melanoma cell line MDA-MB-435S was purchased from the Cell Bank of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). Cell lines were cultured in DMEM growth media (Life Technologies, Carlsbad, CA, USA) which was supplemented with 10% fetal bovine serum (FBS, Life Technologies) and maintained at 37°C in a humidified atmosphere at 5% CO₂. DCA and 2-MeOE2 were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Zhao H., Jiang H., Li Z., Zhuang Y., Liu Y., Zhou S., Xiao Y., Xie C., Zhou F, & Zhou Y. (2017). 2-Methoxyestradiol enhances radiosensitivity in radioresistant melanoma MDA-MB-435R cells by regulating glycolysis via HIF-1α/PDK1 axis. International Journal of Oncology, 50(5), 1531-1540.

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Hypoxia-induced EPO-Luc Reporter Assay

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Cells were stably transfected with plasmid Epo-Luc plasmid. EPOhypoxia response element (HRE)-luciferase reporter plasmid contains three copies of the HRE consensus sequence from the promoter of the erythropoietin gene in the pGL3 vector. Cells (1 × 10 4 ) were seeded the day before the assay. The next day, the cells were stimulated with the test compounds at different concentrations (100, 50, 25, 12.5, 6.25, 3.125, 1563, 0.781, 0.391, and 0.95 μM). 2-MeOE2 ( ≥ 99 %; Sigma-Aldrich) was used as a positive control at a concentration of 0.5 µM. After 72 h of stimulation in hypoxic conditions (1 % O 2 ), the cells were lysed in 25 mM Tris-phosphate pH 7.8, 8 mM MgCl 2 , 1 mM DTT, 1 % Triton X-100, and 7 % glycerol during 15 min at RT in a horizontal shaker. Luciferase activity was measured using a GloMax 96 microplate luminometer (Promega) following the instructions of the luciferase assay kit (Promega). The RLU was calculated and the results are expressed as percentage of inhibition induction/inhibition of EPO-luc activity. Experiments for each concentration of the test items were done in triplicate wells.

Ticona L.A., Cano-Adamuz N., Șerban A.M., & Sánchez Á.R. (2020). Inhibition of HIF-1α through Suppression of NF-κB Activation by Compounds Isolated from Senecio graveolens. Planta Medica International Open, 7(01), e1-e11.

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