Ethylene tetra acetic

Chemicals and materials used throughout this work were obtained from Sigma Chemicals Co., United States) and were used as received without further purification. Analytical grade solvents ethanol, methanol, acetonitrile, trifluoroacetic acid, and formic acid were procured from Merck & Co., Darmstadt, Germany. Sodium chloride, potassium dihydrogen phosphate, glucose, calcium chloride, sodium biphosphate, potassium chloride, magnesium sulfate, sodium bicarbonate, magnesium chloride, and ethylene tetra-acetic acid were sodium bicarbonate, and ethylene tetra-acetic were procured from Merck & Co, Darmstadt, Germany. Acetylcholine chloride, atropine sulfate, verapamil hydrochloride, and L-NAME were procured from Sigma Chemicals, Co., St. Louis, MO, United States. The analytical grade Apigenin-7-O-Glucoside, Catechin, Chlorogenic acid, Ellagic acid, EpiCatechin, Gallic acid, Naringenin, Naringenin-7-O-glucoside, Orientin were procured from Sigma Chemicals, Co., St. Louis, MO, United States.

Analytical grade solvents ethanol, methanol, acetonitrile, trifluoroacetic acid, and formic acid were procured from Merck & Co., Darmstadt, Germany. Sodium chloride, potassium dihydrogen phosphate, glucose, calcium chloride, sodium biphosphate, potassium chloride, magnesium sulfate, sodium bicarbonate, magnesium chloride, and ethylene tetra-acetic acid were sodium bicarbonate, and ethylene tetra-acetic were procured from Merck & Co, Germany. Acetylcholine chloride, atropine sulfate, loperamide hydrochloride, verapamil hydrochloride, carbachol chloride were procured from Sigma Chemicals, Co., St. Louis, MO, USA. The analytical grade umbelliferone, stigmasterol, caffeic acid, quinic acid, rutin ferulic acid, epicatechin, scopoletin, 1,4-dicaffeoylquinic acid, quercetin, apigenin-7-O-glucuronide, hesperidin, malic acid, p-coumaric acid, luteolin 7-O-glucoside and kaempferol were procured from Sigma Chemicals, Co., St. Louis, MO, USA.