Although visualization of vaginal cell characteristics provide strong indication of circulating sex hormones (in particular, E2), additional quantification of serum E2 was performed using enzyme‐linked immunosorbent assay (ELISA; Dougherty et al., 2017a (link); Zabka et al., 2005 (link), 2006 (link)). Briefly, blood samples were collected at the conclusion of phrenic neurophysiology experiments, before urethane overdose. Samples were centrifuged at 845 x g for 10 min, and serum was collected for storage at −80°C. The concentration of E2 was determined using ELISAs (Calbiotech, Spring Valley, CA, USA) according to the manufacturer's instructions; all serum samples were run in triplicate. The absorbance was read at 450 nm, and serum concentrations were interpolated from standard curves run in each assay.
Elisas
Manufactured by Calbiotech
Sourced in United States
ELISAs (Enzyme-Linked Immunosorbent Assays) are a type of analytical biochemistry technique used to detect and quantify specific substances, such as proteins, hormones, or antibodies, in a sample. ELISAs utilize antibodies and color change to identify and measure the target analyte.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using elisas
1
Quantification of Serum Estradiol Levels
2
Quantifying Circulating Estradiol Levels
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