Diminazene aceturate (DA) was acquired from Selleckchem (USA). Interleukin-1β (IL-1β) (# 401-ML) was acquired from R&D systems (USA). Erastin (S7242) was acquired from Selleckchem (USA). ROS assay kit (S0033) was provided by Beyotime (China). Sat1 (10708-1-AP, diluted 1:5000), Nrf2 (U16396-1-AP, diluted 1:5,000), HO-1 (10701-1-AP, diluted 1:5,000), type II collagen (collagen II) (28459-1-AP, diluted 1:2,000), matrix metalloproteinase 13 (MMP13) (18165-1-AP, diluted 1:5,000), MMP3 (17873-1-AP, diluted 1:5000), inducible nitric oxide synthase (iNOS) (22226-1-AP, diluted 1:5000), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibodies (60004-1-Ig, diluted 1:5,000) were acquired from Proteintech (USA). Alox15 (A6865, diluted 1:5000) and lpcat3 antibodies (A17604, diluted 1:1000) were acquired from Abclonal (China). Glutathione peroxidase 4 (GPX4) (ab125066, diluted 1:5,000), SLC7A11 (ab175186, diluted 1:5,000), and NAD(P)H:quinone oxidoreductase 1 (NQO1) antibodies (ab80588, diluted 1:1,000) were acquired from Abcam (UK). The C11 BODIPY Sensor (D3861) was acquired from Thermo Fisher Scientific (USA). The Micro Malondialdehyde (MDA) assay kit (BC0025) was acquired from Solarbio (China).
Slc7a11 ab175186
Manufactured by Abcam
Sourced in United Kingdom
SLC7A11 (ab175186) is a recombinant protein that can be used for research purposes. It is the protein encoded by the SLC7A11 gene, which is involved in the transport of amino acids. This product is intended for use in various research applications, but no further details or interpretations on its intended use can be provided.
Automatically generated - may contain errors
Lab products found in correlation
Erastin, by Selleck Chemicals (2 mentions)
Mmp13, by Proteintech (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody, by Proteintech (1 mentions)
Interleukin 1β il 1β, by R&D Systems (1 mentions)
Micro malondialdehyde mda assay kit, by Solarbio (1 mentions)
Ros assay kit, by Beyotime (1 mentions)
Sb242084, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Nitrocellulose filter membrane, by GE Healthcare (1 mentions)
β actin ab8226, by Abcam (1 mentions)
Radioimmunoprecipitation assay buffer, by Beyotime (1 mentions)
3 protocols using slc7a11 ab175186
1
Diminazene aceturate and oxidative stress
2
Ferroptosis Inducers and Inhibitors Protocol
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Erastin (S7242), RSL3 (S8155), ferrostatin-1 (S7243), liproxstatin-1 (S7699), Z-VAD-FMK (S7023), necrosulfonamide (S8251), staurosporine (S1421), CCT137690 (S2744), olanzapine (S2493), idebenone (S2605), telmisartan (S1738), nisoldipine (S1748), azelnidipine (S3053), and risperidone (S1615) were obtained from Selleck Chemicals (Houston, TX). Cerulein (C9026), L-arginine (A5006), alcohol (PHR1373), 2-mercaptoethanol (M6250), trypsin (T1426), soybean trypsin inhibitor (650357), SB204741 (S0693), and SB242084 (5.06417) were obtained from Sigma-Aldrich (Burlington, MA). Dimethyl sulfoxide (472301; Sigma-Aldrich) was used to prepare the stock solution of most drugs. The final concentration of Dimethyl sulfoxide in the drug working solution in the cells was less than 0.01%. In addition, 0.01% Dimethyl sulfoxide was used as a vehicle control in the corresponding cell culture assays.
The antibodies to PSMD4 (sc-398033) and actin beta (ACTB, sc-8432) were obtained from Santa Cruz Biotechnology (Dallas, TX). The antibodies to GPX4 (ab125066) and SLC7A11 (ab175186) were obtained from Abcam (Cambridge, MA). The antibodies to ferritin heavy chain 1 (3998) and ARNTL (14020) were obtained from Abcam. The antibodies to SLC40A1 (NBP1-21502) and apoptosis inducing factor mitochondria-associated 2 (H00084883-D01P) were obtained from NOVUS (Saint Charles, MO).
The antibodies to PSMD4 (sc-398033) and actin beta (ACTB, sc-8432) were obtained from Santa Cruz Biotechnology (Dallas, TX). The antibodies to GPX4 (ab125066) and SLC7A11 (ab175186) were obtained from Abcam (Cambridge, MA). The antibodies to ferritin heavy chain 1 (3998) and ARNTL (14020) were obtained from Abcam. The antibodies to SLC40A1 (NBP1-21502) and apoptosis inducing factor mitochondria-associated 2 (H00084883-D01P) were obtained from NOVUS (Saint Charles, MO).
3
Western Blot Analysis of Osteoblast Markers
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hFob1.19 cells were washed with PBS and lysed for 30 min in radioimmunoprecipitation assay buffer (Beyotime Biotechnology). Cells were centrifuged at 12,000 × g for 30 min at 4°C, and the supernatant was collected. The protein concentration was measured using the bicinchoninic acid method. Approximately 30 μg of protein was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to nitrocellulose filter membranes (General Electric, Chicago, IL, USA). After blocking, the membranes were incubated with the appropriate primary antibodies at a dilution ratio of 1 : 1000 and overnight at 4°C. The membranes were then incubated with the corresponding secondary antibodies (at a dilution ratio of 1 : 4000) (Cell Signaling Technology, Danvers, USA) at room temperature for 2 h. The protein bands were detected using the EC3 Imaging System (UVP; Analytik Jena AG, Jena, Germany), and the relative intensity of protein bands was quantified using ImageJ software (National Institutes of Health, Bethesda, MD, USA). The following primary antibodies were used: ATF3 (ab254268), GPX4 (ab125066), osteocalcin (OCN) (ab133612), osteoprotegerin (OPG) (ab73400), SLC7A11 (ab175186), and β-actin (ab8226), which were all purchased from Abcam (Cambridge, UK).
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