Tsc22d3

Manufactured by Thermo Fisher Scientific

Tsc22d3 is a laboratory equipment product. It serves a core function within the scientific research and testing workflows. The detailed technical specifications and intended use of this product are not available for this response.

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Lab products found in correlation

Viia 7 real time pcr system, by Thermo Fisher Scientific (1 mentions) Random hexamer, by Promega (1 mentions) Bcl 2, by Thermo Fisher Scientific (1 mentions) Rnaseout inhibitor, by Thermo Fisher Scientific (1 mentions) Tri reagent, by Merck Group (1 mentions) Nanodrop, by Thermo Fisher Scientific (1 mentions) Multiscribe rt, by Thermo Fisher Scientific (1 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (1 mentions)

2 protocols using tsc22d3

Quantitative RT-PCR for Gene Expression Analysis

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Quantitative real-time PCR (qRT-PCR) was used for the confirmation of microarray
data and for quantifying the expression level of Nr4a3 in HUVECs. Briefly,
first-strand cDNA was generated from RNA samples by a 20 μL mixture containing
SuperScript™ III RT (Invitrogen, Bleiswijk, the Netherlands), RNase Out
inhibitor (40 units; Invitrogen) and 250 ng random hexamers (Promega, Leiden,
the Netherlands). 10 ng of cDNA was used for each PCR reaction. Assays were
performed on a ViiA 7 real-time PCR System (Applied Biosystems, Nieuwerkerk aan
den IJssel, The Netherlands) using the absolute QPCR Rox Mix (Thermo Fischer
Scientific). Samples were normalized with ΔCt method using GAPDH as a reference.
Fold change in gene expression versus control was analyzed by the
2^−ΔΔCt method.^{13 (link)} Rat GAPDH (assay ID
Rn01775763_g1), Nars (assay ID Rn01491242_m1), Mars (assay ID Rn01504657_m1),
Yars (assay ID Rn01749701_m1), Gars (assay ID Rn01410234_m1), Iars (assay ID
Rn01450644_m1), Bcl2 (assay ID Rn99999125_m1), Nqo1 (assay ID Rn00566528_m1),
Nqo1 (assay ID Rn00566528_m1), NR4A3 (assay ID Rn01534012_m1), Gpd1 (assay ID
Rn00573596_m1), Stc1 (assay ID Rn00579636_m1) and Tsc22d3 (assay ID
Rn00580222_m1) were purchased as Assay-on-Demand from Applied Biosystems
(Nieuwekerk a/d IJssel, the Netherlands).

Li Y., Faiz A., Moshage H., Schilling L, & Kamps J.A. (2023). Responses of retinal and brain microvasculature to streptozotocin induced diabetes revealed by global expression profiling. Diabetes & Vascular Disease Research, 20(1), 14791641221147533.

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Transcription Profiling of Mammary Fat Tissue

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Total RNA was extracted from the flash-frozen tumor-adjacent and tumor-distal mammary fat pad samples collected at end of study using TRI-Reagent (Sigma-Aldrich, St.Louis, MO) according to manufacturer’s instructions. RNA concentration was spectrophotometrically determined using a nanodrop (Thermo Scientific, Logan, UT) and quality was confirmed using an Agilent 2100 Bioanalyzer (Santa Clara, CA). RNA was reverse transcribed with Multiscribe RT (Applied Biosystems, Carlsbad, CA). Resulting cDNA from tissue samples were assayed in triplicate for PCR using Taqman® Gene Expression Assays for IL-6, TNFα, MMP-9, IL-1β, IGFBP6, CITED1, TFE3, JAK3, EZH2, SMYD3, and TSC22D3 (Applied Biosystems). PCR reactions were completed using a ViiATM7 Real time PCR system (Applied Biosciences). Gene expression data were normalized to the housekeeping gene β-actin and analyzed using the delta delta cycle threshold method (18 (link)).

Rossi E.L., de Angel R.E., Bowers L.W., Khatib S.A., Smith L.A., Van Buren E., Bhardwaj P., Giri D., Estecio M.R., Troester M.A., Hair B.Y., Kirk E.L., Gong T., Shen J., Dannenberg A.J, & Hursting S.D. (2016). Obesity-associated alterations in inflammation, epigenetics and mammary tumor growth persist in formerly obese mice. Cancer prevention research (Philadelphia, Pa.), 9(5), 339-348.

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