Wild type c57bl 6 j jax 000664

YAP^flox/TAZ^flox (JAX 030532), Lyz2-Cre (JAX 004781), and wild-type C57BL/6 J (JAX 000664) mice were purchased from Jackson Laboratory (Indianapolis, IN). Macrophage-specific YAP knockout mice were generated using the Cre/Loxp system. In general, YAP^flox/flox mice were crossbred with lysozyme2 (Lyz2)-Cre mice. Genotypes for the macrophages specific deletion of YAP were confirmed by PCR analysis using the following primers: YAP F: 5′ CCC AAA TTT GAA TCA TTG GGG TCT TTG C 3′; YAP R1: AAC AAA ACC TGG GGA ACG ACT GGG CAC T 3′; YAP R2: GTG CAT AGC TGC ATA ACT TCG TAT AAT GT 3′. Four hundred and ten base pairs for flox allele (F-R1), 315 bp for wild-type allele (F-R1), and 230 bp floxed (deleted) allele (F-R2). Cre gene expression was also examined by PCR. All mice were maintained and bred at the Division of Laboratory Animal Resources at East Tennessee State University (ETSU). All experimental procedures were performed in accordance with the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health (NIH Publication, 8th Edition, 2011) and approved by the ETSU Committee on Animal Care. Age- and sex-matched 8–12 weeks mice were used for all experiments. Mice were randomly allocated into each treatment group. Investigators were blinded to group allocation during data collection and analysis.

YAP^flox (JAX 030532), Lyz2-Cre (JAX 004781) and wildtype C57BL/6J (JAX 000664) mice were purchased from Jackson Laboratory (Indianapolis, IN). Macrophage specific YAP knockout mice were generated using the Cre/Loxp system. In general, YAP^flox/flox mice were crossbred with lysozyme2 (Lyz2)-Cre mice. Genotypes for the macrophages specific deletion of YAP were confirmed by PCR analysis using the following primers: YAP F: 5′ CCC AAA TTT GAA TCA TTG GGG TCT TTG C 3′; YAP R1: AAC AAA ACC TGG GGA ACG ACT GGG CAC T 3′; YAP R2: GTG CAT AGC TGC ATA ACT TCG TAT AAT GT 3′. 410 bp for flox allele (F-R1), 315 bp for wild type allele (F-R1) and 230 bp floxed (deleted) allele (F-R2). Cre gene expression was also examined by PCR. The experiments outlined in this manuscript conform to the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health (NIH Publication, 8^th Edition, 2011). The experimental protocols were approved by the ETSU Committee on Animal Care.