Comprehensive diagnostic profile rotor

Manufactured by Abaxis

Sourced in United States

The Comprehensive Diagnostic Profile rotors are a component of Abaxis' lab equipment designed to perform a range of diagnostic tests. These rotors are used in conjunction with Abaxis' analytical instruments to provide comprehensive diagnostic information, but without further details on their specific functions or intended uses.

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Lab products found in correlation

Vetscan vs2, by Abaxis (3 mentions) Vetscan vs2 chemistry analyzer, by Abaxis (2 mentions) Vetscan 2, by Abaxis (1 mentions) Vetscan hm5 hematology analyzer, by Abaxis (1 mentions) Hemavet 950 system, by Drew Scientific (1 mentions) Microvette 500 lh, by Sarstedt (1 mentions) Tissue tek oct compound, by Sakura Finetek (1 mentions) Hemavet 950, by Drew Scientific (1 mentions) Scn400 slide scanner, by Leica camera (1 mentions)

8 protocols using comprehensive diagnostic profile rotor

Hematological and Biochemical Profiling

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Blood was collected in EDTA tubes to analyze common hematology parameters using a HEMAVET 950 Multispecies Hematology Analyzer (Drew Scientific, Miami Lakes, FL, USA). Measured parameters included total white blood cell counts (WBC), total and percent neutrophils (NE), lymphocytes (LY), monocytes (MO), eosinophils (EO), basophils (BA), and monocytes (MO). Additional blood volume was collected in SST tubes, processed to serum, and analyzed for changes in clinical chemistry using a VetScan VS2 Chemistry Analyzer and Comprehensive Diagnostic Profile rotors (Abaxis, Union City, CA, USA) as previously described [39 (link)]. Samples collected from uninfected animals euthanized on day 0 served as baseline.

Escaffre O., Juelich T.L., Neef N., Massey S., Smith J., Brasel T., Smith J.K., Kalveram B., Zhang L., Perez D., Ikegami T., Freiberg A.N, & Comer J.E. (2021). STAT-1 Knockout Mice as a Model for Wild-Type Sudan Virus (SUDV). Viruses, 13(7), 1388.

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Comprehensive Hematology and Blood Chemistry Analysis

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Hematology was performed on an HM5 analyzer (Abaxis, https://www.abaxis.com) by using K3 EDTA-treated whole blood. We evaluated erythrocytes, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, red cell distribution weight, platelets, mean platelet volume, leukocytes, and absolute and percent neutrophil count, lymphocyte count, monocyte count, eosinophil count, and basophil count. Blood chemistries were evaluated on a VetScan 2 (Abaxis) with Comprehensive Diagnostic Profile rotors (Abaxis) by using serum stored at −80°C until tested. We evaluated glucose, blood urea nitrogen, creatinine, calcium, albumin, total protein, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, amylase, potassium, sodium, phosphate, chloride, globulin, and total bilirubin. We used sodium heparin-treated blood to analyze venous blood gases by using an iSTAT Alinity V (Abaxis) instrument with a CG4+ cartridge (Abaxis) to measure lactate, pH, total carbon dioxide, partial pressure carbon dioxide, partial pressure oxygen, soluble oxygen, bicarbonate, and base excess. We used age-specific values and the instrument reference intervals to establish normal ranges (18 (link)–20 (link)).

Pickering B.S., Smith G., Pinette M.M., Embury-Hyatt C., Moffat E., Marszal P, & Lewis C.E. (2021). Susceptibility of Domestic Swine to Experimental Infection with Severe Acute Respiratory Syndrome Coronavirus 2. Emerging Infectious Diseases, 27(1), 104-112.

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Comprehensive Biomarker Profiling for Viral Infection

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Femoral vein peripheral blood was collected via Vacutainer® into standard collection tubes containing a clot activator (serum separator tubes) or EDTA. Clinical chemistry analyses were conducted on harvested serum using the Abaxis VetScan VS2® Chemistry Analyzer using Comprehensive Diagnostic Profile rotors (Abaxis, Inc., Union City, CA, USA). Hematology was performed on EDTA blood using the Abaxis VETSCAN® HM5 Hematology Analyzer (Abaxis, Inc., Union City, CA, USA). Serum was frozen for viral load analysis. Nasal, oral, and rectal swabs were collected on days 0–5, 7, 10, and 14 using sterile cotton-tipped medical swabs which were placed into 0.5 mL sterile phosphate-buffered saline (PBS) for viral load analysis.

Johnson D.M., Brasel T., Massey S., Garron T., Grimes M., Smith J., Torres M., Wallace S., Villasante-Tezanos A., Beasley D.W, & Comer J.E. (2022). Evaluation of molnupiravir (EIDD-2801) efficacy against SARS-CoV-2 in the rhesus macaque model. Antiviral Research, 209, 105492.

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Serum Biochemistry Analysis for Toxicity

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Serum biochemistry was analyzed for 14 biochemical markers of general toxicity (listed in supplemental table 1) to determine exposure effects. All procedures followed manufacturer’s protocols. On day of analysis, random samples of serum were thawed at 4°C and 120μL/sample added to the Comprehensive Diagnostic profile rotors (500–7123; Abaxis, Union City, CA). For quality assurance, a control sample (pooled from 20 random samples) was ran approximately every 25 samples. Rotors were placed in the VetScan VS2 Whole Blood chemistry analyzer and data exported. Final numbers were controls (13F,14M), BFR (11F,9M), OPFR (12F,11M) and FM550 (14F,15M). Although 21 statistical outliers from 6 biomarkers across female and male groups were identified, all data was used because no animal had greater than 2 outlying samples across all biomarkers.

Witchey S.K., Samara L.A., Horman B.M., Stapleton H.M, & Patisaul H.B. (2020). Perinatal exposure to FireMaster® 550 (FM550), brominated or organophosphate flame retardants produces sex and compound specific effects on adult Wistar rat socioemotional behavior. Hormones and behavior, 126, 104853.

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Evaluating Tranilast Safety in Mice

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In order to determine if tranilast administration was safe, we tested the plasma collected from the mice after 8 weeks of treatment with the vehicle or tranilast. The plasma (100 μL) was pipetted into the Comprehensive Diagnostic Profile Rotor (#500-1038, Abaxis, Union City, CA, USA), and the values of glucose, creatinine, BUN, phosphorus, sodium, albumin, alkaline phosphatase, serum glutamic pyruvic transaminase (SGPT; ALT), total protein, globulin, total bilirubin, and amylase were analyzed using the VetScan VS2 chemistry analyzer (Abaxis, Union City, CA, USA).

Chuang T.D., Munoz L., Quintanilla D., Boos D, & Khorram O. (2023). Therapeutic Effects of Long-Term Administration of Tranilast in an Animal Model for the Treatment of Fibroids. International Journal of Molecular Sciences, 24(13), 10465.

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Hematological and Serum Biochemical Analysis

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To determine hematological parameters, whole blood in K₂EDTA tubes was analyzed using the Hemavet 950 system (Drew Scientific Inc., Miami Lakes, FL, USA) according to manufacturer instructions. For serum biochemical parameters, serum was harvested from SST tubes and clarified by centrifugation (5 min, 6000× g). Serum was immediately aliquoted and stored at −80 °C for PRNT and Bioplex assays. At least 200 μL from each NHP was not frozen and inoculated immediately into a Comprehensive Diagnostic Profile rotor (Abaxis, Union City, CA, USA) and subjected to biochemical analysis via the VetScan VS2 (Abaxis, Union City, CA, USA) according to manufacturer instructions.

Azar S.R., Rossi S.L., Haller S.H., Yun R., Huang J.H., Plante J.A., Zhou J., Olano J.P., Roundy C.M., Hanley K.A., Weaver S.C, & Vasilakis N. (2018). ZIKV Demonstrates Minimal Pathologic Effects and Mosquito Infectivity in Viremic Cynomolgus Macaques. Viruses, 10(11), 661.

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Liver Analysis in Mouse Model

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Liver was harvested when mice reached the indicated timepoints and fixed in 10% neutral buffered formalin (NBF) for histology, frozen in Tissue-Tek OCT compound (Sakura Finetek) in liquid nitrogen-cooled isopentane for cryosections, or snap frozen in liquid nitrogen for RNA and protein extraction. Blood was collected by cardiac puncture, kept in lithium heparin-coated Microvette 500 LH (20.1345.100; Sarstedt), and analysed using the Comprehensive Diagnostic Profile rotor (500–0038; Abaxis) on the Vetscan VS2 (Abaxis) for liver function, or using the Hemavet 950 (Drew Scientific) for blood leukocyte counting. Embryonic day 14.5 (E14.5) hepatoblasts and postnatal day 14 (P14) hepatocytes were isolated using a previously published protocol [78 ].

Dewhurst M.R., Ow J.R., Zafer G., van Hul N.K., Wollmann H., Bisteau X., Brough D., Choi H, & Kaldis P. (2020). Loss of hepatocyte cell division leads to liver inflammation and fibrosis. PLoS Genetics, 16(11), e1009084.

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In Vivo Toxicity Assessment of Nanoparticles

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BALB/c female mice10–12 weeks of age were intravenously injected with 100 μL of PBS or ZHS-QDs in PBS (18 nmol Zn per g body weight) followed 30 min later with intraperitoneal injection of 300 μL of PBS or 1x Ag-TS. For biodistribution and clearance studies, the mice were sacrificed under deep anesthesia 1 h, 24 h, and 10 days after Ag-TS injection. Major tissues, serum, feces and urine samples were collected for quantification of mercury with ICP-OES^{42 (link)}. To examine in vivo toxicity, blood was collected from the retro-orbital plexus of each mouse under deep anesthesia into a lithium-heparin 1.3 ml micro tube (Sarstedt, Nümbrecht, Germany) after 24 h and 1 week, and plasma was isolated by centrifugation. The plasma (100 μl) was pipetted into a Comprehensive Diagnostic Profile Rotor (Product Part No. 500-1038, Abaxis, Union City, CA) and subjected to biochemical toxicity assays using an AbaxisVetScan VS2 chemistry analyzer. The mice were then sacrificed under deep anesthesia, and tissues were collected and processed for paraffin embedding. Sectioned paraffin blocks were stained with hematoxylin and eosin (H&E), and whole slide scanning was performed with a Leica SCN 400 slide scanner.

Liu X., Braun G.B., Qin M., Ruoslahti E, & Sugahara K.N. (2017). In vivo cation exchange in quantum dots for tumor-specific imaging. Nature Communications, 8, 343.

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