Pcdna3.1 malat1

miR-144-3p mimics negative control (NC), miR-144-3p mimics, miR-144-3p inhibitor negative control (NC), miR-144-3p inhibitor, pcDNA3.1, and pcDNA3.1-MALAT1 were synthesized by RiboBio (Guangzhou, China). ShMALAT1 negative control (NC), shMALAT1, shNRF2, and shNRF2 negative control (NC) were purchased from GenPharma (Shanghai, China). When the confluence of HLE-B3 cells that were cultured in DMEM with 5.5 mM glucose reached 70%–80%, the cells were transfected with above RNA fragment by Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA) for 36 h and then were stimulated with 30 mM glucose for 24 h.

The primary endometrial stromal cells (ESCs) were obtained according to previous description. ESCs were cultured in Dulbecco's Modified Eagle Medium (DMEM, Gibco, USA) with 10% fetal bovine serum (FBS, Gibco, USA), 10 U/ml penicillin, and 100U/ml streptomycin at 37°C and 5% CO₂.
ESCs (1х10⁵ cells/mL) were cultured in a 24-well plate for 24 h. The miR-206 mimic, NC-mimic, miR-206 inhibitor, and NC-inhibitor were obtained from GenePharma (Shanghai, China). pcDNA3.1-MALAT1 and pcDNA3.1-MALAT1 negative control (pcDNA-NC) were obtained from RIBO Bio (Guangzhou, China). Lipofectamine 3000 transfection kit (Invitrogen, USA) was used for cell transfection according to the transfection instructions.