Clone 17f3

Manufactured by BioXCell

The Clone 17F3 is a laboratory instrument designed for cell culture and cell line manipulation. It provides a controlled environment for the growth and maintenance of cells. The core function of this product is to facilitate cell culture operations.

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Lab products found in correlation

Clone xmg1, by BioXCell (1 mentions) Il 4 clone 11b11, by BioXCell (1 mentions) Clone r4 6a2, by BioXCell (1 mentions) C57bl 6j female mice, by Jackson ImmunoResearch (1 mentions) Clone 2a3, by BioXCell (1 mentions) Clone mopc 21, by BioXCell (1 mentions) Af3239, by R&D Systems (1 mentions)

5 protocols using clone 17f3

Cytokine Blockade in Hypophysitis

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To assess the role of IFN-γ and IL-17A in the pathophysiology of hypophysitis, antibodies neutralizing IFN-γ (clone XMG1.2) or IL-17A (clone 17F3, both from Bio X Cell) were injected in the peritoneum three times at a dose of 500 μg/mouse (a total of 1.5 mg/mouse). The injections were performed on days −1, 6, 14 (to investigate the effect of blocking the cytokines before disease onset/early in the disease process) or on days 11, 15, 19 (after disease induction).

Chalan P., Thomas N, & Caturegli P. (2021). Th17 cells contribute to the pathology of autoimmune hypophysitis.. Journal of immunology (Baltimore, Md. : 1950), 206(11), 2536-2543.

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Cytokine Neutralization for CCA Tumor Model

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IFNγ, IL4, or IL17a neutralization was performed by i.p. injections of 100 µg of rat IgG1 antibodies targeting IFNγ (clone R4-6A2; BioXCell), IL4 (clone 11B11; BioXcell), or IL17a (clone 17F3; BioXCell) at the following time points: 3 d and 1 d before CCA cell engraftment, then 1 d after, then repeated three times a week throughout the whole course of the experiment. Isotype control (clone HRPN; BioXCell) was delivered i.p. at the same dose and time points.

Paillet J., Plantureux C., Lévesque S., Le Naour J., Stoll G., Sauvat A., Caudana P., Tosello Boari J., Bloy N., Lachkar S., Martins I., Opolon P., Checcoli A., Delaune A., Robil N., de la Grange P., Hamroune J., Letourneur F., Autret G., Leung P.S., Gershwin M.E., Zhu J.S., Kurth M.J., Lekbaby B., Augustin J., Kim Y., Gujar S., Coulouarn C., Fouassier L., Zitvogel L., Piaggio E., Housset C., Soussan P., Maiuri M.C., Kroemer G, & Pol J.G. (2021). Autoimmunity affecting the biliary tract fuels the immunosurveillance of cholangiocarcinoma. The Journal of Experimental Medicine, 218(10), e20200853.

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Cytokine-Induced Bone Remodeling in Mice

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Female C57BL/6J mice (The Jackson Laboratory) at 10 weeks of age were randomly assigned to receive control antibody (100 μg; clone 2A3, catalog BE0089), RANKL (100 μg; clone IK22/5, catalog BE0191), TNF-α (100 μg; clone TN3-19.12, catalog BE0244), or IL-17a (200 μg; clone 17F3, catalog BP0173) (all purchased from BioXcell) via i.p. injection twice a week. After 5 weeks on the Pi diets, mice were sacrificed, and bones, RNA, and serum were harvested for analysis.

Roberts J.L., Yu M., Viggeswarapu M., Arnst J.L., Pacifici R, & Beck GR J.r. (2023). Dietary phosphorus consumption alters T cell populations, cytokine production, and bone volume in mice. JCI Insight, 8(10), e154729.

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Modulating Retinal Vascularization in OIR

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P12 mice pups were anesthetized in 3% isoflurane with oxygen and injected intravitreally with 2 μl of CM, whereas basal αMEM (vehicle) was injected in the contralateral eye as control using a Hamilton syringe equipped with 50-gage glass capillary. 2 μl of mouse recombinant Sema3E Fc Chimera (20 ng/μl; R&D systems) or PBS were administered into vitreous cavity of P12 OIR pups. To assess the effect of blocking IL-17A on the retinal vascularization, 2 μl of 5 μg/μl neutralizing monoclonal IL-17A antibody (Clone 17F3, BioXCell) or of 5 μg/μl mouse IgG1 isotype control monoclonal antibody (Clone MOPC-21, BioXCell) was intravitreally injected. To reverse the benefits of Sema3E induced by MSCs-CM administration during OIR, the eyes were injected at P12 and P14 with 2 μl of blocking Sema3E (5 μg/μl) antibody (AF3239, R&D Systems). To assess the dose-response of MSCs on vasoobliteration, 50,000, 100,000, and 200, 000 cells from passages 3–5 were intravitreally injected in a volume of 2 μl. Retinal vasculature was analyzed in whole-mounts at P17.

Noueihed B., Rivera J.C., Dabouz R., Abram P., Omri S., Lahaie I, & Chemtob S. (2021). Mesenchymal Stromal Cells Promote Retinal Vascular Repair by Modulating Sema3E and IL-17A in a Model of Ischemic Retinopathy. Frontiers in Cell and Developmental Biology, 9, 630645.

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Intracerebroventricular Administration of BDNF, IL-17 Inhibitors in Mice

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WT and IL17 -/- mice were administered BDNF (0.1 mg/mL, total volume of 3 μl), saline solution (PBS, total volume of 3 μl) or anti-mouse IL-17 (0.8 mg/mL, total volume of 5 μl) (Clone 17F3, BioXCell) and IgG1 isotype control (0.8 mg/mL, total volume of 5 μl) (Clone MOPC-21) by i.c.v. injection as previously described (35 (link)). Briefly, mice were anesthetized under 1.5% isoflurane in 100% oxygen in a transparent acrylic chamber. After the induction, mice were moved out of the chamber to a stereotaxic frame, and isoflurane anesthesia was maintained. A single i.c.v was performed into the right ventricle of the brain, using the stereotaxic coordinates of 0.6 mm posterior, 1.2 mm lateral and 2.2 mm ventral to Bregma. A 10 μl hamilton syringe was used for i.c.v injection. Behavioral assessment was performed 24h after surgery in the Y-maze.

Ribeiro M., Brigas H.C., Temido-Ferreira M., Pousinha P.A., Regen T., Santa C., Coelho J.E., Marques-Morgado I., Valente C., Omenetti S., Stockinger B., Waissman A., Manadas B., Lopes L.V., Silva-Santos B, & Ribot J.C. (2019). Meningeal γδ T cell-derived IL-17 controls synaptic plasticity and short-term memory. Science immunology, 4(40), eaay5199.

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