Penicillin sodium

Manufactured by Merck Group

Sourced in United States

Penicillin sodium is a pharmaceutical product used as an active ingredient in various antibiotic medications. It is a white, crystalline powder that is soluble in water and commonly used in medical and laboratory settings.

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Close spelling variants of this product

Penicillin (7834 citations) Penicillin G sodium salt (49 citations) Penicillin G sodium (25 citations) Penicillin sodium salt (2 citations)

7 protocols using penicillin sodium

Atorvastatin Pharmacokinetics and Biomarkers

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QS (Lot No. 20170504) was provided by Beijing Xiusheng Pharmaceutical Co. Atorvastatin calcium tablets (Pfizer Pharmaceuticals Ltd., Lot No. S56741), IL-2 (Lot No. R20210328), IL-6 (Lot No. R20210328), TNF-α (Lot No. R20210328), creatine kinase isozyme cardiac type (CK-MB) (Lot No. R20210327), and cardiac troponin I (cTnI) (Lot No. R20210327) kits were purchased from the Nanjing Jiancheng Institute of Biological Engineering. Sodium pentobarbital (2%), lidocaine, sodium chloride (0.9%), sodium penicillin, methoxyamine HCl, fatty acid methyl ester (C7-C30, FAMEs) standards, pyridine, and anhydrous sodium sulfate were obtained from Sigma-Aldrich (St. Louis, MO, USA). MSTFA (N-methyl-N-(trimethylsilyl)trifluoroacetamide) with 1% (vol/vol) trimethylchlorosilane (MSTFA, with 1% TMCS), methanol (Optima LC-MS), acetonitrile (Optima LC-MS), hexane, dichloromethane, chloroform, and acetone were purchased from Thermo-Fisher Scientific (FairLawn, NJ, USA). Ultrapure water was produced by a Mill-Q Reference system equipped with an LC-MS Pak filter (Millipore, Billerica, MA).

Zhao N., Ma Y., Liang X., Zhang Y., Hong D., Wang Y, & Bai D. (2023). Efficacy and Mechanism of Qianshan Huoxue Gao in Acute Coronary Syndrome via Regulation of Intestinal Flora and Metabolites. Drug Design, Development and Therapy, 17, 579-595.

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Isolation and Characterization of Equine MSCs

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MSCs were collected and assessed via routine processing techniques^{20 (link)} using bone marrow aspirate collected from the sternum of the same four donor horses as the DTS material. Cells were cultured at 37°C, 5% CO₂ and 90% humidity in standard MSC media: low-glucose GlutaMAX DMEM with 110μg/mL sodium pyruvate (Gibco, USA) plus 10% MSC FBS (Gibco, USA) and 100U/mL sodium penicillin, 100μg/mL streptomycin sulfate (Sigma, USA). Cells were expanded in monolayer culture to 80% confluence and passaged twice. Flow cytometry was conducted on these four separate cell lines with a BD FACSCalibur using monoclonal antibodies previously validated in our laboratory (data not shown). The results demonstrated that approximately 90% of cells were positive for CD-90 and CD-44, common MSC surface antigens, as well as Oct-4, which is indicative of a naïve stemness found in both embryonic^{21 (link)} and adult²² stem cell populations.

Youngstrom D.W., Rajpar I., Kaplan D.L, & Barrett J.G. (2015). A bioreactor system for in vitro tendon differentiation and tendon tissue engineering. Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 33(6), 911-918.

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Culturing Human Gastric Cancer SNU-1 Cells

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Human gastric cancer cell line SNU-1 was grown in Roswell Park Memorial Institute (RPMI)-1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA), 100 U/ml penicillin sodium, and 100 μg/ml streptomycin sulfate (Sigma-Aldrich, St. Louis, MO USA) at 37°C in a humidified atmosphere of 5% CO₂14 (link).

Li C., Li X., Gao S., Li C, & Ma L. (2017). MicroRNA-133a Inhibits Proliferation of Gastric Cancer Cells by Downregulating ERBB2 Expression. Oncology Research, 25(7), 1169-1176.

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Cell Culture of Various Cell Lines

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All cell lines were routinely cultured in a humidified incubator at 37°C under 5% CO₂ in the indicated culture medium containing 10% fetal bovine serum (FBS, Sigma Aldrich) supplemented with 100 U/ml penicillin sodium and 100 μg/ml streptomycin sulfate (Sigma Aldrich).
HEK293T, HeLa (ATCC, American Type Culture Collection), and DAOY cells (kind gift from Dr. Maike Glitsch, Department of Physiology, Anatomy and Genetics, University of Oxford, UK) were grown in Dulbecco's modified Eagle's medium (DMEM, Gibco/Invitrogen), while PC3 cells (kindly provided by Dr. Richard Bryant from Ludwig Institute for Cancer Research, University of Oxford, UK) were maintained in RPMI 1640 (Lonza). Ptch1^+/+, Ptch1^+/−, and Ptch1^−/− mouse embryonic fibroblasts (MEFs, kind gift from Dr. Philip Beachy, Department of Biochemistry, Stanford University), Sufu^−/− MEFs (kindly provided by Dr. Stephan Teglund, Department of Biosciences and Nutrition, Karolinska Institutet, Sweden), and NIH3T3 cells (ATCC) were cultured in DMEM supplemented with 1 mM sodium pyruvate (Sigma Aldrich) and 0.1 mM MEM nonessential amino acids (Sigma Aldrich).

Raducu M., Fung E., Serres S., Infante P., Barberis A., Fischer R., Bristow C., Thézénas M., Finta C., Christianson J.C., Buffa F.M., Kessler B.M., Sibson N.R., Di Marcotullio L., Toftgård R, & D'Angiolella V. (2016). SCF (Fbxl17) ubiquitylation of Sufu regulates Hedgehog signaling and medulloblastoma development. The EMBO Journal, 35(13), 1400-1416.

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Determination of 5-FU Purity

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5-FU(purity >99 %) was purchased from Chengdu Hua Xia chemical reagent co., LTD, vitaminc(Vc)(purity >99.7 %), 2,6-ditert-butyl-4-methylphenol(BHT)(purity >99.9 %) and Penicillin sodium were purchased from Sigma-Aldrich. Acetonitrile was obtained from Merck. The solvents used for HPLC (high performance liquid chromatography) were of HPLC grade. All other chemicals and reagents used in this study were of analytical grade.

Yin L., Lu Q., Tan S., Ding L., Guo Y., Chen F, & Tang L. (2016). Bioactivity-guided isolation of antioxidant and anti-hepatocarcinoma constituents from Veronica ciliata. Chemistry Central Journal, 10, 27.

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Synthesis and Characterization of Liguzinediol

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Liguzinediol (purity > 98%) was provided and synthesized by Professor Wei Li from Nanjing University of Chinese Medicine. The structure was elucidated and confirmed in the previous study [8 (link)]. The international patent protection of liguzinediol has been applied (No. PCT/CN2009/075100). Captopril and Digoxin were purchased from Shanghai Sine Pharmaceutical Co., Ltd (Shanghai, China). Sodium chloride, heparin, chloral hydrate, and penicillin sodium were obtained from Sigma-Aldrich (St. Louis, USA). All Elisa kits were purchased from MultiSciences (Lianke) Biotech Co., Ltd. (Hangzhou, China), and all antibodies were obtained from Abcam (Cambridge, UK).

Chen Q., Zhang D., Bi Y., Zhang W., Zhang Y., Meng Q., Li Y, & Bian H. (2020). The protective effects of liguzinediol on congestive heart failure induced by myocardial infarction and its relative mechanism. Chinese Medicine, 15, 63.

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Liguzinediol Synthesis and Characterization

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Liguzinediol (purity > 98%) was provided and synthesized by Professor Wei Li from Nanjing University of Chinese Medicine. The structure was elucidated and con rmed in the previous study [8] . The international patent protection of liguzinediol has been applied (No. PCT/CN2009/075100). Captopril and Digoxin were purchased from Shanghai Sine Pharmaceutical Co., Ltd (Shanghai, China). Sodium chloride, heparin, chloral hydrate, and penicillin sodium were obtained from Sigma-Aldrich (St. Louis, USA). All Elisa kits were purchased from MultiSciences (Lianke) Biotech Co., Ltd. (Hangzhou, China), and all antibodies were obtained from Abcam (Cambridge, UK).

Chen Q., Zhang D., Bi Y., Zhang W., Zhang Y., Meng Q., Yu H., & Bian H. (2020). The protective effects of liguzinediol on congestive heart failure induced by myocardial infarction and its relative mechanism.

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