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Gm130 rabbit mab

Manufactured by Cell Signaling Technology
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GM130 rabbit mAb is a primary antibody product manufactured by Cell Signaling Technology. It is specific for the detection of the GM130 protein, which is a cis-Golgi matrix protein involved in the maintenance of Golgi structure and function.

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Lab products found in correlation

Lamp2, by Santa Cruz Biotechnology (1 mentions) Gm130 antibody, by BD (1 mentions) Golgin 97, by Proteintech (1 mentions) Golgin97, by Santa Cruz Biotechnology (1 mentions) Lamp1 rabbit mab, by Cell Signaling Technology (1 mentions) Phospho sting s366, by Cell Signaling Technology (1 mentions) F7425, by Merck Group (1 mentions) Flag m2, by Merck Group (1 mentions) Gapdh, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Lsm710 system, by Zeiss (1 mentions) Hsp60 rabbit mab, by Cell Signaling Technology (1 mentions) α tubulin rabbit mab, by Cell Signaling Technology (1 mentions) Plan apochromat 63 1.40 na oil diciii objective lens, by Zeiss (1 mentions) Alexa fluor 594 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Rabbit mABs GM130

2 protocols using gm130 rabbit mab

1

Antibody Characterization for Cellular Organelles

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Antibodies for LAMP1 (sc-20011, IF 1:200); LAMP2 (sc-18822, IF 1:200); Golgin97 (sc-59820, IF 1:500); GAPDH (sc-365062, WB 1:5000); Tubulin (sc-5286, WB 1:3000) were from Santa Cruz Biotechnology. The GM130 antibody (610822, IF 1:1000) was from BD Biosciences. Flag (M2, IF 1:1000; F7425, WB 1:3000) antibody was from Sigma. LAMP1 rabbit mAb (#9091, IF 1:200); GM130 rabbit mAb (#12480, IF 1:300); Rab5 rabbit mAb (#3547, IF 1:200), Phospho-STING S366 (#50907, WB 1:1000); Phospho-TBK1 Ser172 rabbit mAb (#5483, WB/IF 1:1000) were from Cell Signaling. The following antibodies were from Proteintech: STING (19851-1-AP, WB 1:1000, IF 1:1000); LC3 (14600-1-AP, IF 1:1000, WB 1:1000, reacts with LC3A, LC3B, and LC3C); TGN46 (13573-1-AP, IF 1:1000); Golgin97 (12640-1-AP, IF 1:1000). Alexa-488/594- and Pacific Blue-conjugated secondary antibodies were obtained from ThermoFisher Scientific.
Xun J., Zhang Z., Lv B., Lu D., Yang H., Shang G, & Tan J.X. (2024). A conserved ion channel function of STING mediates noncanonical autophagy and cell death. EMBO Reports, 25(2), 544-569.
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2

Visualizing Intracellular Protein Localization

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To visualize intracellular proteins, immunofluorescence staining was applied using primary antibodies and appropriate fluorescent secondary antibodies. The following primary antibodies were used: Acetyl-alpha Tubulin (Lys40) mouse mAb (1:100, Thermo Fisher Scientific), α-tubulin rabbit mAb (1:100, Cell signaling technology), GM-130 rabbit mAb (1:100, Cell signaling technology), Pericentrin rabbit pAb (1:100, Abcam), Hsp60 rabbit mAb (1:100, Cell signaling technology). The following secondary antibodies were used: Alexa Fluor 594 goat anti-mouse IgG (1:200, Thermo Fisher Scientific, A-11032), Alexa Fluor 488 goat anti-rabbit IgG (1:200, Thermo Fisher Scientific, A-11008). Nuclei were stained with DAPI. The LSM710 system (Carl Zeiss) with a Plan Apochromat 63 × /1.40-NA oil DICIII objective lens (Carl Zeiss) was used to acquire images.
Wu B., Qiu J., Zhao T.V., Wang Y., Maeda T., Goronzy I.N., Akiyama M., Ohtsuki S., Jin K., Tian L., Goronzy J.J, & Weyand C.M. (2020). Succinyl-CoA Ligase Deficiency in Pro-inflammatory and Tissue-Invasive T Cells. Cell metabolism, 32(6), 967-980.e5.
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